Chemopreventive and biological activities of Helicteres isora L. fruit extracts
© 2018 Medknow Publications. All rights reserved. Helicteres isora L. (H. isora) has been used in traditional medicine in Asia. This study was aimed to determine biological activities of H. isora fruit extracts. Chemopreventive effect was examined by cell proliferation assay and differentiation-ind...
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th-mahidol.473332019-08-28T13:54:16Z Chemopreventive and biological activities of Helicteres isora L. fruit extracts Acharaporn Rattanamaneerusmee Krit Thirapanmethee Yasushi Nakamura Bhanubong Bongcheewin Mullika Traidej Chomnawang Mahidol University Kyoto Prefectural University Forestry and Fisheries Technology Center Pharmacology, Toxicology and Pharmaceutics © 2018 Medknow Publications. All rights reserved. Helicteres isora L. (H. isora) has been used in traditional medicine in Asia. This study was aimed to determine biological activities of H. isora fruit extracts. Chemopreventive effect was examined by cell proliferation assay and differentiation-inducing effect. Anti-inflammatory activity of extracts was studied on the levels of nitric oxide (NO), tumor necrosis factor alpha (TNF-α), production of prostaglandin E2 (PGE-2), and cyclooxygenas-2 (COX-2). Cell proliferation assay revealed that H. isora extracts and its major compound, rosmarinic acid, showed no cytotoxicity in THP-1 and RCM-1 cells. Methylthio acetic acid from Cucumis melo var.conomon used as a positive control and 80% ethanol extracts demonstrated significant cell differentiation induction. Hexane extract of H. isora could lower the levels of TNF-α, PGE-2, and NO in THP-1 cells with 51.61 ± 0.79%, 69.68 ± 0.017%, and 69.93 ± 9.41% inhibition, respectively. The highest inhibitory effect on COX-2 was obtained from dichloromethane extract. Dexamethasone inhibited the secretion of TNF-α with 95.82 ± 0.50% while celecoxib showed the inhibitory effect on COX-2 and PGE-2 with 100% and 99.86%, respectively. The ethanol extract showed the best antioxidant activity by DPPH and FRAP assays at IC 50 of 5.43 ± 1.01 μg/mL and 22.83 ± 0.13 mmol FeSO 4 /g sample, respectively, while the positive control, trolox, showed the antioxidant activity with IC 50 and FRAP values at 4.08 ± 0.85 μg/mL and 10.84 ± 0.04 mmol FeSO 4 /g sample, respectively. Taken together, H. isora possess chemopreventive and antioxidant activity. Further studies on in vivo activities of this plant are suggested. 2019-08-28T06:54:16Z 2019-08-28T06:54:16Z 2018-01-01 Article Research in Pharmaceutical Sciences. Vol.13, No.6 (2018), 476-483 10.4103/1735-5362.245960 17359414 17355362 2-s2.0-85058615524 https://repository.li.mahidol.ac.th/handle/123456789/47333 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85058615524&origin=inward |
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Pharmacology, Toxicology and Pharmaceutics Acharaporn Rattanamaneerusmee Krit Thirapanmethee Yasushi Nakamura Bhanubong Bongcheewin Mullika Traidej Chomnawang Chemopreventive and biological activities of Helicteres isora L. fruit extracts |
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© 2018 Medknow Publications. All rights reserved. Helicteres isora L. (H. isora) has been used in traditional medicine in Asia. This study was aimed to determine biological activities of H. isora fruit extracts. Chemopreventive effect was examined by cell proliferation assay and differentiation-inducing effect. Anti-inflammatory activity of extracts was studied on the levels of nitric oxide (NO), tumor necrosis factor alpha (TNF-α), production of prostaglandin E2 (PGE-2), and cyclooxygenas-2 (COX-2). Cell proliferation assay revealed that H. isora extracts and its major compound, rosmarinic acid, showed no cytotoxicity in THP-1 and RCM-1 cells. Methylthio acetic acid from Cucumis melo var.conomon used as a positive control and 80% ethanol extracts demonstrated significant cell differentiation induction. Hexane extract of H. isora could lower the levels of TNF-α, PGE-2, and NO in THP-1 cells with 51.61 ± 0.79%, 69.68 ± 0.017%, and 69.93 ± 9.41% inhibition, respectively. The highest inhibitory effect on COX-2 was obtained from dichloromethane extract. Dexamethasone inhibited the secretion of TNF-α with 95.82 ± 0.50% while celecoxib showed the inhibitory effect on COX-2 and PGE-2 with 100% and 99.86%, respectively. The ethanol extract showed the best antioxidant activity by DPPH and FRAP assays at IC 50 of 5.43 ± 1.01 μg/mL and 22.83 ± 0.13 mmol FeSO 4 /g sample, respectively, while the positive control, trolox, showed the antioxidant activity with IC 50 and FRAP values at 4.08 ± 0.85 μg/mL and 10.84 ± 0.04 mmol FeSO 4 /g sample, respectively. Taken together, H. isora possess chemopreventive and antioxidant activity. Further studies on in vivo activities of this plant are suggested. |
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Mahidol University |
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Mahidol University Acharaporn Rattanamaneerusmee Krit Thirapanmethee Yasushi Nakamura Bhanubong Bongcheewin Mullika Traidej Chomnawang |
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Article |
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Acharaporn Rattanamaneerusmee Krit Thirapanmethee Yasushi Nakamura Bhanubong Bongcheewin Mullika Traidej Chomnawang |
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Acharaporn Rattanamaneerusmee |
title |
Chemopreventive and biological activities of Helicteres isora L. fruit extracts |
title_short |
Chemopreventive and biological activities of Helicteres isora L. fruit extracts |
title_full |
Chemopreventive and biological activities of Helicteres isora L. fruit extracts |
title_fullStr |
Chemopreventive and biological activities of Helicteres isora L. fruit extracts |
title_full_unstemmed |
Chemopreventive and biological activities of Helicteres isora L. fruit extracts |
title_sort |
chemopreventive and biological activities of helicteres isora l. fruit extracts |
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2019 |
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https://repository.li.mahidol.ac.th/handle/123456789/47333 |
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1763498194467028992 |