Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.

The 1st Pharmaceutical Sciences Asia Conference 2019 Theme : Pharmaceutical Sciences toward Health Innovation in the Disruptive Era. Bangkok Midtown Hotel, Thailand. August 22, 2019, page 26-27

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Main Authors:	Pornpatsorn Lertphadungkit, Somnuk Bunsupa
Other Authors:	Mahidol University. Faculty of Pharmacy. Department of Pharmaceutical Chemistry
Format:	Proceeding Abstract
Language:	English
Published:	2020
Subjects:	Trichosanthes cucumerina L. bryonolic acid plant tissue cultures and elicitors
Online Access:	https://repository.li.mahidol.ac.th/handle/123456789/49498
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Institution:	Mahidol University
Language:	English

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spelling	th-mahidol.494982023-03-31T01:41:08Z Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L. Pornpatsorn Lertphadungkit Somnuk Bunsupa Mahidol University. Faculty of Pharmacy. Department of Pharmaceutical Chemistry Trichosanthes cucumerina L. bryonolic acid plant tissue cultures and elicitors The 1st Pharmaceutical Sciences Asia Conference 2019 Theme : Pharmaceutical Sciences toward Health Innovation in the Disruptive Era. Bangkok Midtown Hotel, Thailand. August 22, 2019, page 26-27 Trichosanthes cucumerina L. (TC) is a Thai medicinal plant belonging to Cucurbitaceae family. This plant has been used in Thai traditional medicine for purgative, emetics, treating dandruff and relieving the sinusitis. Additionally, it has been reported about its activities, especially cytotoxicity and many studies showed that these activities related with a compound named bryonolic acid. This compound is one of major triterpenoid compounds that can be generally found in TC root. This study aimed to improve bryonolic acid content using plant tissue culture techniques for the study of genes in bryonolic acid biosynthesis. The callus was induced using Murashige and Skoog (MS) plant media supplemented with 0.5 mg/ml of indole- 3-butyric acid (IBA) and 1 mg/ml of benzylaminopurine (BA). For the cell suspensions, they were cultured in MS media with 1 mg/ml of IBA and 1 mg/ml of BA. Three elicitors, methyl jasmonate (50 - 200 μM), yeast extract (0.1 - 2% w/v) and chitosan (1 - 100 mg/ml), were added into the cell suspension. The results from HPLC analysis showed that callus and cell suspension presented the higher bryonolic acid levels by just over twenty and seven times compared with TC root (approximately 2 mg/g dry weight), respectively. In the presence of elicitors, the best treated condition was to treat cell suspensions with 1 mg/ml of chitosan for 8 days (approximately 24 mg/g dry weight). To carry out the candidate genes in bryonolic acid biosynthesis pathway, RNA was extracted from TC calli for the de novo transcriptome analysis compared with TC leaf and fruit to carry out the candidate genes associated with bryonolic acid biosynthetic pathway. 2020-01-24T09:17:03Z 2020-01-24T09:17:03Z 2020-01-24 2019 Proceeding Abstract https://repository.li.mahidol.ac.th/handle/123456789/49498 eng Mahidol University Faculty of Pharmacy Mahidol University application/pdf
institution	Mahidol University
building	Mahidol University Library
continent	Asia
country	Thailand Thailand
content_provider	Mahidol University Library
collection	Mahidol University Institutional Repository
language	English
topic	Trichosanthes cucumerina L. bryonolic acid plant tissue cultures and elicitors
spellingShingle	Trichosanthes cucumerina L. bryonolic acid plant tissue cultures and elicitors Pornpatsorn Lertphadungkit Somnuk Bunsupa Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.
description	The 1st Pharmaceutical Sciences Asia Conference 2019 Theme : Pharmaceutical Sciences toward Health Innovation in the Disruptive Era. Bangkok Midtown Hotel, Thailand. August 22, 2019, page 26-27
author2	Mahidol University. Faculty of Pharmacy. Department of Pharmaceutical Chemistry
author_facet	Mahidol University. Faculty of Pharmacy. Department of Pharmaceutical Chemistry Pornpatsorn Lertphadungkit Somnuk Bunsupa
format	Proceeding Abstract
author	Pornpatsorn Lertphadungkit Somnuk Bunsupa
author_sort	Pornpatsorn Lertphadungkit
title	Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.
title_short	Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.
title_full	Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.
title_fullStr	Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.
title_full_unstemmed	Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.
title_sort	transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in trichosanthes cucumerina l.
publishDate	2020
url	https://repository.li.mahidol.ac.th/handle/123456789/49498
_version_	1763491023350136832

Transcriptome analysis and identification of genes associated with bryonolic acid biosynthesis in Trichosanthes cucumerina L.

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