The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor

© 2019, Springer Science+Business Media, LLC, part of Springer Nature. To address the high demand for Pueraria candollei var. mirifica (PM) used as the active ingredient in health products and its difficulty to cultivate in the field, the growth and production of deoxymiroestrol (DME) and isoflavono...

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Main Authors: Orapin Udomsin, Gorawit Yusakul, Tharita Kitisripanya, Thaweesak Juengwatanatrakul, Waraporn Putalun
Other Authors: Ubon Rajathanee University
Format: Article
Published: 2020
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/49558
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spelling th-mahidol.495582020-01-27T10:34:03Z The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor Orapin Udomsin Gorawit Yusakul Tharita Kitisripanya Thaweesak Juengwatanatrakul Waraporn Putalun Ubon Rajathanee University Walailak University Khon Kaen University Mahidol University Biochemistry, Genetics and Molecular Biology Chemical Engineering Immunology and Microbiology © 2019, Springer Science+Business Media, LLC, part of Springer Nature. To address the high demand for Pueraria candollei var. mirifica (PM) used as the active ingredient in health products and its difficulty to cultivate in the field, the growth and production of deoxymiroestrol (DME) and isoflavonoid (ISF) phytoestrogens in PM cell suspensions were studied. In a 125-mL shake flask, the cell suspension produced DME [78.7 ± 8.79–116 ± 18.2 μg/g dry weight (DW)] and ISF (140 ± 6.83–548 ± 18.5 μg/g DW), which are the predominant ISF glycosides. While ISF aglycones accumulated in the PM cell suspension cultured in the airlift bioreactor. The DME content was increased to 976 ± 79.6 μg/g DW when the PM cell suspension was cultured in the 5-L scale bioreactor. The production of DME and ISF was enhanced by elicitors including methyl jasmonate (MJ), yeast extract (YE), and chitosan (CHI). MJ produced the highest induction of DME accumulation, while ISF accumulation was the highest with YE treatment. Analysis of catalase activity implied that the elicitors enhanced ROS production, which resulted in the enhancement of DME and ISF production and accumulation in PM cell suspension cultures. PM cell suspension culture is a promising source of beneficial PM phytoestrogens that exhibit bioactivity that may useful for the treatment of menopausal symptoms. 2020-01-27T03:30:24Z 2020-01-27T03:30:24Z 2020-01-01 Article Applied Biochemistry and Biotechnology. Vol.190, No.1 (2020), 57-72 10.1007/s12010-019-03094-y 15590291 02732289 2-s2.0-85069688925 https://repository.li.mahidol.ac.th/handle/123456789/49558 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069688925&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Immunology and Microbiology
Orapin Udomsin
Gorawit Yusakul
Tharita Kitisripanya
Thaweesak Juengwatanatrakul
Waraporn Putalun
The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor
description © 2019, Springer Science+Business Media, LLC, part of Springer Nature. To address the high demand for Pueraria candollei var. mirifica (PM) used as the active ingredient in health products and its difficulty to cultivate in the field, the growth and production of deoxymiroestrol (DME) and isoflavonoid (ISF) phytoestrogens in PM cell suspensions were studied. In a 125-mL shake flask, the cell suspension produced DME [78.7 ± 8.79–116 ± 18.2 μg/g dry weight (DW)] and ISF (140 ± 6.83–548 ± 18.5 μg/g DW), which are the predominant ISF glycosides. While ISF aglycones accumulated in the PM cell suspension cultured in the airlift bioreactor. The DME content was increased to 976 ± 79.6 μg/g DW when the PM cell suspension was cultured in the 5-L scale bioreactor. The production of DME and ISF was enhanced by elicitors including methyl jasmonate (MJ), yeast extract (YE), and chitosan (CHI). MJ produced the highest induction of DME accumulation, while ISF accumulation was the highest with YE treatment. Analysis of catalase activity implied that the elicitors enhanced ROS production, which resulted in the enhancement of DME and ISF production and accumulation in PM cell suspension cultures. PM cell suspension culture is a promising source of beneficial PM phytoestrogens that exhibit bioactivity that may useful for the treatment of menopausal symptoms.
author2 Ubon Rajathanee University
author_facet Ubon Rajathanee University
Orapin Udomsin
Gorawit Yusakul
Tharita Kitisripanya
Thaweesak Juengwatanatrakul
Waraporn Putalun
format Article
author Orapin Udomsin
Gorawit Yusakul
Tharita Kitisripanya
Thaweesak Juengwatanatrakul
Waraporn Putalun
author_sort Orapin Udomsin
title The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor
title_short The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor
title_full The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor
title_fullStr The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor
title_full_unstemmed The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor
title_sort deoxymiroestrol and isoflavonoid production and their elicitation of cell suspension cultures of pueraria candollei var. mirifica: from shake flask to bioreactor
publishDate 2020
url https://repository.li.mahidol.ac.th/handle/123456789/49558
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