PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon
© 2019 Elsevier Ltd Yellow head disease (YHD) is an infectious disease of Penaeus monodon which is caused by the yellow head virus (YHV). YHV infection invariably leads to 100% shrimp mortality within 3–5 days. Currently, an effective method to prevent or cure shrimp from YHV infection has not been...
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th-mahidol.496992020-01-27T15:43:30Z PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon Pratsaneeyaporn Posiri Sudarat Thongsuksangcharoen Nattawadee Chaysri Sakol Panyim Chalermporn Ongvarrasopone Mahidol University Thailand National Center for Genetic Engineering and Biotechnology Agricultural and Biological Sciences Environmental Science © 2019 Elsevier Ltd Yellow head disease (YHD) is an infectious disease of Penaeus monodon which is caused by the yellow head virus (YHV). YHV infection invariably leads to 100% shrimp mortality within 3–5 days. Currently, an effective method to prevent or cure shrimp from YHV infection has not been elucidated. Therefore, the molecular mechanism underlying YHV infection should be examined. In this study, early endosome antigen 1 (EEA1) protein that was involved in the tethering step of the vesicle and early endosome fusion was investigated during YHV infection. The open reading frame of P. monodon EEA1 (PmEEA1) was cloned and sequenced (3000 bp). It encoded a putative protein of 999 amino acids and contained the zinc finger C2H2 domain signature at the N-terminus and the FYVE domain at the C-terminus. Suppression of PmEEA1 by specific dsRNA in shrimp showed inhibition of YHV replication after 48 h post YHV injection (hpi). On the other hand, shrimp received only NaCl without any dsRNA showed high YHV levels at approximately one hundred thousand times at 24 hpi and 48 hpi. Moreover, silencing of PmEEA1 by specific dsRNA followed by YHV challenge demonstrated a delay in shrimp mortality from 60 hpi to 168 hpi when compared to the control. These results indicated that YHV required PmEEA1 for trafficking within the infected cells, strongly suggesting that PmEEA1 may be a potential target to control and prevent YHV infection in P. monodon. 2020-01-27T07:20:50Z 2020-01-27T07:20:50Z 2019-12-01 Article Fish and Shellfish Immunology. Vol.95, (2019), 449-455 10.1016/j.fsi.2019.10.054 10959947 10504648 2-s2.0-85075426942 https://repository.li.mahidol.ac.th/handle/123456789/49699 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85075426942&origin=inward |
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Agricultural and Biological Sciences Environmental Science Pratsaneeyaporn Posiri Sudarat Thongsuksangcharoen Nattawadee Chaysri Sakol Panyim Chalermporn Ongvarrasopone PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon |
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© 2019 Elsevier Ltd Yellow head disease (YHD) is an infectious disease of Penaeus monodon which is caused by the yellow head virus (YHV). YHV infection invariably leads to 100% shrimp mortality within 3–5 days. Currently, an effective method to prevent or cure shrimp from YHV infection has not been elucidated. Therefore, the molecular mechanism underlying YHV infection should be examined. In this study, early endosome antigen 1 (EEA1) protein that was involved in the tethering step of the vesicle and early endosome fusion was investigated during YHV infection. The open reading frame of P. monodon EEA1 (PmEEA1) was cloned and sequenced (3000 bp). It encoded a putative protein of 999 amino acids and contained the zinc finger C2H2 domain signature at the N-terminus and the FYVE domain at the C-terminus. Suppression of PmEEA1 by specific dsRNA in shrimp showed inhibition of YHV replication after 48 h post YHV injection (hpi). On the other hand, shrimp received only NaCl without any dsRNA showed high YHV levels at approximately one hundred thousand times at 24 hpi and 48 hpi. Moreover, silencing of PmEEA1 by specific dsRNA followed by YHV challenge demonstrated a delay in shrimp mortality from 60 hpi to 168 hpi when compared to the control. These results indicated that YHV required PmEEA1 for trafficking within the infected cells, strongly suggesting that PmEEA1 may be a potential target to control and prevent YHV infection in P. monodon. |
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Mahidol University |
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Mahidol University Pratsaneeyaporn Posiri Sudarat Thongsuksangcharoen Nattawadee Chaysri Sakol Panyim Chalermporn Ongvarrasopone |
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Article |
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Pratsaneeyaporn Posiri Sudarat Thongsuksangcharoen Nattawadee Chaysri Sakol Panyim Chalermporn Ongvarrasopone |
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Pratsaneeyaporn Posiri |
title |
PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon |
title_short |
PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon |
title_full |
PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon |
title_fullStr |
PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon |
title_full_unstemmed |
PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon |
title_sort |
pmeea1, the early endosomal protein is employed by yhv for successful infection in penaeus monodon |
publishDate |
2020 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/49699 |
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1763490211598172160 |