Characterization and probiotic properties of Lactobacilli from human breast milk
© 2019, King Abdulaziz City for Science and Technology. Several studies have reported a complex microbial community in human breast milk. This community impacts the shape of the infant gut microbiota and consequently impacts host health. Lactobacillus is an important probiotic and has many applicati...
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th-mahidol.497122020-01-27T15:43:35Z Characterization and probiotic properties of Lactobacilli from human breast milk Chanettee Jamyuang Phanphen Phoonlapdacha Nalinee Chongviriyaphan Wasaporn Chanput Sunee Nitisinprasert Massalin Nakphaichit Kasetsart University Faculty of Medicine, Ramathibodi Hospital, Mahidol University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Environmental Science © 2019, King Abdulaziz City for Science and Technology. Several studies have reported a complex microbial community in human breast milk. This community impacts the shape of the infant gut microbiota and consequently impacts host health. Lactobacillus is an important probiotic and has many applications in the functional food industry. This study isolated and evaluated the potential probiotic bacteria from human milk. Two Lactobacillus species, L. plantarum and L. pentosus, were isolated from the breast milk of Thai women. L. pentosus HM04-22, L. pentosus HM04-3, L. plantarum HM04-80, L. plantarum HM04-88 and L. plantarum HM01-1 showed good adhesion activity (> 55%) and resistance in gastric (pH 2) and bile (pH 8) conditions. Characterization of the probiotic properties indicated that all selected Lactobacillus isolates had anti-adhesion properties against Escherichia coli and Salmonella Typhimurium. Lactobacillus isolates protected Caco-2 cells from pathogen adhesion at 25–40%. In addition, the five selected strains presented anti-inflammatory properties by reducing interleukin (IL)-8 expression at 0.14 ± 0.16 to 0.52 ± 0.117-fold. However, the strains had no effect on the expression of tight junction genes, including zona occludens (ZO)-1, occludin and claudin-1. In conclusion, five selected Lactobacillus isolates from human milk were candidates for use as probiotics to promote health. However, more tests in animal models and clinical trials need to be performed. 2020-01-27T07:21:08Z 2020-01-27T07:21:08Z 2019-11-01 Article 3 Biotech. Vol.9, No.11 (2019) 10.1007/s13205-019-1926-y 21905738 2190572X 2-s2.0-85073523754 https://repository.li.mahidol.ac.th/handle/123456789/49712 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85073523754&origin=inward |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Environmental Science Chanettee Jamyuang Phanphen Phoonlapdacha Nalinee Chongviriyaphan Wasaporn Chanput Sunee Nitisinprasert Massalin Nakphaichit Characterization and probiotic properties of Lactobacilli from human breast milk |
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© 2019, King Abdulaziz City for Science and Technology. Several studies have reported a complex microbial community in human breast milk. This community impacts the shape of the infant gut microbiota and consequently impacts host health. Lactobacillus is an important probiotic and has many applications in the functional food industry. This study isolated and evaluated the potential probiotic bacteria from human milk. Two Lactobacillus species, L. plantarum and L. pentosus, were isolated from the breast milk of Thai women. L. pentosus HM04-22, L. pentosus HM04-3, L. plantarum HM04-80, L. plantarum HM04-88 and L. plantarum HM01-1 showed good adhesion activity (> 55%) and resistance in gastric (pH 2) and bile (pH 8) conditions. Characterization of the probiotic properties indicated that all selected Lactobacillus isolates had anti-adhesion properties against Escherichia coli and Salmonella Typhimurium. Lactobacillus isolates protected Caco-2 cells from pathogen adhesion at 25–40%. In addition, the five selected strains presented anti-inflammatory properties by reducing interleukin (IL)-8 expression at 0.14 ± 0.16 to 0.52 ± 0.117-fold. However, the strains had no effect on the expression of tight junction genes, including zona occludens (ZO)-1, occludin and claudin-1. In conclusion, five selected Lactobacillus isolates from human milk were candidates for use as probiotics to promote health. However, more tests in animal models and clinical trials need to be performed. |
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Kasetsart University |
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Kasetsart University Chanettee Jamyuang Phanphen Phoonlapdacha Nalinee Chongviriyaphan Wasaporn Chanput Sunee Nitisinprasert Massalin Nakphaichit |
format |
Article |
author |
Chanettee Jamyuang Phanphen Phoonlapdacha Nalinee Chongviriyaphan Wasaporn Chanput Sunee Nitisinprasert Massalin Nakphaichit |
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Chanettee Jamyuang |
title |
Characterization and probiotic properties of Lactobacilli from human breast milk |
title_short |
Characterization and probiotic properties of Lactobacilli from human breast milk |
title_full |
Characterization and probiotic properties of Lactobacilli from human breast milk |
title_fullStr |
Characterization and probiotic properties of Lactobacilli from human breast milk |
title_full_unstemmed |
Characterization and probiotic properties of Lactobacilli from human breast milk |
title_sort |
characterization and probiotic properties of lactobacilli from human breast milk |
publishDate |
2020 |
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https://repository.li.mahidol.ac.th/handle/123456789/49712 |
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1763492918123823104 |