Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp

• Main Authors: Thawatchai Chaijarasphong, Thanyawit Thammachai, Ornchuma Itsathitphaisarn, Kallaya Sritunyalucksana, Rungkarn Suebsing
• Other Authors: Mahidol University
• Format: Article
• Published: 2020
• Subjects: Agricultural and Biological Sciences
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/49717

© 2019 Elsevier B.V. White spot disease (WSD), caused by white spot syndrome virus (WSSV), is among the most severe diseases of cultivated shrimp. Here, the CRISPR-Cas12a system coupled with nucleic acid amplification was optimized for the detection of WSSV. The CRISPR-Cas12a system was used to spec...