Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis

© 2018, The Society for In Vitro Biology. Phytostilbenes are responsible for several biological activities of mulberry (Morus sp.), which has been widely used as a raw material in health products. This study aimed to investigate the capability of Morus alba L. cell in bioreactors to produce the majo...

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Main Authors: Jukrapun Komaikul, Tharita Kitisripanya, Chadathorn Inyai, Kittisak Likhitwitayawuid, Boonchoo Sritularak, Hiroyuki Tanaka, Waraporn Putalun
Other Authors: Chulalongkorn University
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Published: 2020
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/49778
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spelling th-mahidol.497782020-01-27T14:45:57Z Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis Jukrapun Komaikul Tharita Kitisripanya Chadathorn Inyai Kittisak Likhitwitayawuid Boonchoo Sritularak Hiroyuki Tanaka Waraporn Putalun Chulalongkorn University Khon Kaen University Mahidol University Kyushu University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology © 2018, The Society for In Vitro Biology. Phytostilbenes are responsible for several biological activities of mulberry (Morus sp.), which has been widely used as a raw material in health products. This study aimed to investigate the capability of Morus alba L. cell in bioreactors to produce the major bioactive stilbenes. The cell obtained from air-driven bioreactors such as round bottom, flat bottom, and air-lift vessel shape bioreactors was collected and analyzed for the levels of mulberroside A and oxyresveratrol. The results showed that the cell culture in round bottom and air-lift vessel bioreactors had higher growth rate, as compared with the cell culture in shake flasks (1.38- and 1.41-fold, respectively). The optimized culture condition to produce mulberroside A was obtained from round bottom bioreactor culture (55.56 ± 11.41 μmol/L). Additionally, endogenous stilbenoid hydrolysis of cell from the bioreactor culture was examined. Under optimized hydrolytic conditions, mulberroside A in the cell was readily deglycosylated to give oxyresveratrol within 1 h. These results indicated that the glycoside mulberroside A in the cell is sensitive to the endogenous enzymatic hydrolysis. Interaction of the stilbenoid components with the endogenous hydrolytic enzyme triggered by cell disruption in M. alba samples was suggested to be the major cause of the alteration of the stilbenoid levels. These findings have provided a new approach to producing glycosidic compounds and corresponding aglycones in cell culture. 2020-01-27T07:24:09Z 2020-01-27T07:24:09Z 2019-04-15 Article In Vitro Cellular and Developmental Biology - Plant. Vol.55, No.2 (2019), 199-208 10.1007/s11627-018-09953-3 10545476 2-s2.0-85062817354 https://repository.li.mahidol.ac.th/handle/123456789/49778 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85062817354&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Jukrapun Komaikul
Tharita Kitisripanya
Chadathorn Inyai
Kittisak Likhitwitayawuid
Boonchoo Sritularak
Hiroyuki Tanaka
Waraporn Putalun
Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis
description © 2018, The Society for In Vitro Biology. Phytostilbenes are responsible for several biological activities of mulberry (Morus sp.), which has been widely used as a raw material in health products. This study aimed to investigate the capability of Morus alba L. cell in bioreactors to produce the major bioactive stilbenes. The cell obtained from air-driven bioreactors such as round bottom, flat bottom, and air-lift vessel shape bioreactors was collected and analyzed for the levels of mulberroside A and oxyresveratrol. The results showed that the cell culture in round bottom and air-lift vessel bioreactors had higher growth rate, as compared with the cell culture in shake flasks (1.38- and 1.41-fold, respectively). The optimized culture condition to produce mulberroside A was obtained from round bottom bioreactor culture (55.56 ± 11.41 μmol/L). Additionally, endogenous stilbenoid hydrolysis of cell from the bioreactor culture was examined. Under optimized hydrolytic conditions, mulberroside A in the cell was readily deglycosylated to give oxyresveratrol within 1 h. These results indicated that the glycoside mulberroside A in the cell is sensitive to the endogenous enzymatic hydrolysis. Interaction of the stilbenoid components with the endogenous hydrolytic enzyme triggered by cell disruption in M. alba samples was suggested to be the major cause of the alteration of the stilbenoid levels. These findings have provided a new approach to producing glycosidic compounds and corresponding aglycones in cell culture.
author2 Chulalongkorn University
author_facet Chulalongkorn University
Jukrapun Komaikul
Tharita Kitisripanya
Chadathorn Inyai
Kittisak Likhitwitayawuid
Boonchoo Sritularak
Hiroyuki Tanaka
Waraporn Putalun
format Article
author Jukrapun Komaikul
Tharita Kitisripanya
Chadathorn Inyai
Kittisak Likhitwitayawuid
Boonchoo Sritularak
Hiroyuki Tanaka
Waraporn Putalun
author_sort Jukrapun Komaikul
title Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis
title_short Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis
title_full Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis
title_fullStr Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis
title_full_unstemmed Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis
title_sort phytostilbenoid production in white mulberry (morus alba l.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis
publishDate 2020
url https://repository.li.mahidol.ac.th/handle/123456789/49778
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