Tissue storage solution for preservation and transfer of forensic specimen in high ambient-temperature
© 2019 Elsevier B.V. Storage of tissue samples in high ambient-temperature can affect the quality of forensic evidence. Experiments were conducted to investigate the potential use of 3 tissue storage solutions for the preservation and transfer of forensic specimen in high ambient temperature conditi...
Saved in:
Main Authors: | , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2020
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/50001 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.50001 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.500012020-01-27T16:17:28Z Tissue storage solution for preservation and transfer of forensic specimen in high ambient-temperature Thant Zin Achirapa Bandhaya Nathinee Panvisavas Mahidol University Biochemistry, Genetics and Molecular Biology Medicine © 2019 Elsevier B.V. Storage of tissue samples in high ambient-temperature can affect the quality of forensic evidence. Experiments were conducted to investigate the potential use of 3 tissue storage solutions for the preservation and transfer of forensic specimen in high ambient temperature conditions, i.e., DMSO, Longmire's buffer, and trehalose solution. Results showed that DNA in tissue was best preserved in DMSO buffer. Samples preserved in Longmire's buffer gave DNA analysis results for temperatures up to 60 °C, however, amplification between replications were not reproducible. For those tissue samples preserved in trehalose solution, DNA markers larger than 300 bp were absent, and irreproducible amplification results were detected at a higher level when the storage temperature increased, and storage time was over 2 weeks. Tissue storage condition at high temperature over 1 week is not recommended. Experimental results here provided an alternative collection and preservation method for tissue samples at ambient temperature (without cold-storage) for subsequent DNA analysis. These can potentially be implemented in forensic biological evidence collection, preservation and transfer in hot climates. 2020-01-27T07:34:36Z 2020-01-27T07:34:36Z 2019-12-01 Article Forensic Science International: Genetics Supplement Series. Vol.7, No.1 (2019), 182-184 10.1016/j.fsigss.2019.09.071 1875175X 18751768 2-s2.0-85073573850 https://repository.li.mahidol.ac.th/handle/123456789/50001 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85073573850&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Biochemistry, Genetics and Molecular Biology Medicine |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Medicine Thant Zin Achirapa Bandhaya Nathinee Panvisavas Tissue storage solution for preservation and transfer of forensic specimen in high ambient-temperature |
description |
© 2019 Elsevier B.V. Storage of tissue samples in high ambient-temperature can affect the quality of forensic evidence. Experiments were conducted to investigate the potential use of 3 tissue storage solutions for the preservation and transfer of forensic specimen in high ambient temperature conditions, i.e., DMSO, Longmire's buffer, and trehalose solution. Results showed that DNA in tissue was best preserved in DMSO buffer. Samples preserved in Longmire's buffer gave DNA analysis results for temperatures up to 60 °C, however, amplification between replications were not reproducible. For those tissue samples preserved in trehalose solution, DNA markers larger than 300 bp were absent, and irreproducible amplification results were detected at a higher level when the storage temperature increased, and storage time was over 2 weeks. Tissue storage condition at high temperature over 1 week is not recommended. Experimental results here provided an alternative collection and preservation method for tissue samples at ambient temperature (without cold-storage) for subsequent DNA analysis. These can potentially be implemented in forensic biological evidence collection, preservation and transfer in hot climates. |
author2 |
Mahidol University |
author_facet |
Mahidol University Thant Zin Achirapa Bandhaya Nathinee Panvisavas |
format |
Article |
author |
Thant Zin Achirapa Bandhaya Nathinee Panvisavas |
author_sort |
Thant Zin |
title |
Tissue storage solution for preservation and transfer of forensic specimen in high ambient-temperature |
title_short |
Tissue storage solution for preservation and transfer of forensic specimen in high ambient-temperature |
title_full |
Tissue storage solution for preservation and transfer of forensic specimen in high ambient-temperature |
title_fullStr |
Tissue storage solution for preservation and transfer of forensic specimen in high ambient-temperature |
title_full_unstemmed |
Tissue storage solution for preservation and transfer of forensic specimen in high ambient-temperature |
title_sort |
tissue storage solution for preservation and transfer of forensic specimen in high ambient-temperature |
publishDate |
2020 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/50001 |
_version_ |
1763492259894919168 |