Urinary metabolic profiles after vitamin D<inf>2</inf> versus vitamin D<inf>3</inf> supplementation in prediabetes

© 2019 The Authors Aim: To assess the potential biological differences between vitamin D2 and D3 using urinary metabolite profiles in response to vitamin D3 or D2 supplementation. Method: Subjects consisted of 29 subjects with impaired fasting glucose and/or impaired glucose tolerance. Subjects were...

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Main Authors: Laor Chailurkit, Hataikarn Nimitphong, Sunee Saetung, Boonsong Ongphiphadhanakul
Other Authors: Faculty of Medicine, Ramathibodi Hospital, Mahidol University
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Published: 2020
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/50157
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spelling th-mahidol.501572020-01-27T16:46:38Z Urinary metabolic profiles after vitamin D<inf>2</inf> versus vitamin D<inf>3</inf> supplementation in prediabetes Laor Chailurkit Hataikarn Nimitphong Sunee Saetung Boonsong Ongphiphadhanakul Faculty of Medicine, Ramathibodi Hospital, Mahidol University Biochemistry, Genetics and Molecular Biology Medicine © 2019 The Authors Aim: To assess the potential biological differences between vitamin D2 and D3 using urinary metabolite profiles in response to vitamin D3 or D2 supplementation. Method: Subjects consisted of 29 subjects with impaired fasting glucose and/or impaired glucose tolerance. Subjects were randomized into two groups, vitamin D2 (20,000 IU weekly, n = 14) or vitamin D3 (15,000 IU weekly, n = 15). Urine and serum samples were taken at two different time points for each subject (at baseline and at 12 weeks). Urinary metabolite profiling was performed by liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS). Serum calcium was analyzed on an automated biochemical analyzer and serum intact parathyroid hormone was determined by electrochemiluminescence immunoassay. Results: At baseline, there was no statistically significant difference in clinical characteristics including age, gender, body mass index, waist circumference and 25-hydroxyvitamin D (25(OH)D) levels between the 2 groups. Weekly administration of 20,000 U D2 for 12 weeks resulted in comparable 25(OH)D concentrations as compared to weekly 15,000 U D3 supplementation (97.8 ± 305 vs. 96.8 ± 3.4 nmol/L, p = 0.84). No difference in serum calcium (2.3 ± 0.03 vs. 2.2 ± 0.03 nmol/L, p = 0.52) or intact parathyroid hormone (5.3 ± 0.3 vs. 4.9 ± 0.5 pmol/L, p = 0.54) at 12 weeks was found. Principle component analysis did not reveal apparent segregation of metabolites according to D2 or D3 supplementation. Moreover, using partial least square regression, no apparent separation between the D2 and the D3 group was found. No important metabolite influencing the separation of the D2 from the D3 group was found using variables importance on projection analysis. Conclusions: At comparable circulating 25(OH)D concentrations, vitamin D2 or D3 supplementation does not appear to result in different urinary metabolite profiles. Our finding does not support a biological difference between vitamin D2 and D3. 2020-01-27T07:43:05Z 2020-01-27T07:43:05Z 2019-06-01 Article Journal of Clinical and Translational Endocrinology. Vol.16, (2019) 10.1016/j.jcte.2019.100194 22146237 2-s2.0-85065781986 https://repository.li.mahidol.ac.th/handle/123456789/50157 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85065781986&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Laor Chailurkit
Hataikarn Nimitphong
Sunee Saetung
Boonsong Ongphiphadhanakul
Urinary metabolic profiles after vitamin D<inf>2</inf> versus vitamin D<inf>3</inf> supplementation in prediabetes
description © 2019 The Authors Aim: To assess the potential biological differences between vitamin D2 and D3 using urinary metabolite profiles in response to vitamin D3 or D2 supplementation. Method: Subjects consisted of 29 subjects with impaired fasting glucose and/or impaired glucose tolerance. Subjects were randomized into two groups, vitamin D2 (20,000 IU weekly, n = 14) or vitamin D3 (15,000 IU weekly, n = 15). Urine and serum samples were taken at two different time points for each subject (at baseline and at 12 weeks). Urinary metabolite profiling was performed by liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS). Serum calcium was analyzed on an automated biochemical analyzer and serum intact parathyroid hormone was determined by electrochemiluminescence immunoassay. Results: At baseline, there was no statistically significant difference in clinical characteristics including age, gender, body mass index, waist circumference and 25-hydroxyvitamin D (25(OH)D) levels between the 2 groups. Weekly administration of 20,000 U D2 for 12 weeks resulted in comparable 25(OH)D concentrations as compared to weekly 15,000 U D3 supplementation (97.8 ± 305 vs. 96.8 ± 3.4 nmol/L, p = 0.84). No difference in serum calcium (2.3 ± 0.03 vs. 2.2 ± 0.03 nmol/L, p = 0.52) or intact parathyroid hormone (5.3 ± 0.3 vs. 4.9 ± 0.5 pmol/L, p = 0.54) at 12 weeks was found. Principle component analysis did not reveal apparent segregation of metabolites according to D2 or D3 supplementation. Moreover, using partial least square regression, no apparent separation between the D2 and the D3 group was found. No important metabolite influencing the separation of the D2 from the D3 group was found using variables importance on projection analysis. Conclusions: At comparable circulating 25(OH)D concentrations, vitamin D2 or D3 supplementation does not appear to result in different urinary metabolite profiles. Our finding does not support a biological difference between vitamin D2 and D3.
author2 Faculty of Medicine, Ramathibodi Hospital, Mahidol University
author_facet Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Laor Chailurkit
Hataikarn Nimitphong
Sunee Saetung
Boonsong Ongphiphadhanakul
format Article
author Laor Chailurkit
Hataikarn Nimitphong
Sunee Saetung
Boonsong Ongphiphadhanakul
author_sort Laor Chailurkit
title Urinary metabolic profiles after vitamin D<inf>2</inf> versus vitamin D<inf>3</inf> supplementation in prediabetes
title_short Urinary metabolic profiles after vitamin D<inf>2</inf> versus vitamin D<inf>3</inf> supplementation in prediabetes
title_full Urinary metabolic profiles after vitamin D<inf>2</inf> versus vitamin D<inf>3</inf> supplementation in prediabetes
title_fullStr Urinary metabolic profiles after vitamin D<inf>2</inf> versus vitamin D<inf>3</inf> supplementation in prediabetes
title_full_unstemmed Urinary metabolic profiles after vitamin D<inf>2</inf> versus vitamin D<inf>3</inf> supplementation in prediabetes
title_sort urinary metabolic profiles after vitamin d<inf>2</inf> versus vitamin d<inf>3</inf> supplementation in prediabetes
publishDate 2020
url https://repository.li.mahidol.ac.th/handle/123456789/50157
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