Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma
© 2019 Elsevier Ltd Objective: This study aimed to determine mitochondrial mRNA expression levels and the relationships between these expression levels and various adverse clinicopathological characteristics. Methods: The mRNA expression levels of all 12 genes encoded protein, located on the heavy-s...
Saved in:
Main Authors: | , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2020
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/50241 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.50241 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.502412020-01-27T17:03:01Z Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma Pitsapaporn Wangpermtam Songsak Petmitr Phaibul Punyarit Boworn Klongnoi Sirima Sanguansin Army Institute of Pathology Bumrungrad International Hospital Mahidol University Biochemistry, Genetics and Molecular Biology Dentistry Medicine © 2019 Elsevier Ltd Objective: This study aimed to determine mitochondrial mRNA expression levels and the relationships between these expression levels and various adverse clinicopathological characteristics. Methods: The mRNA expression levels of all 12 genes encoded protein, located on the heavy-strand of mitochondrial DNA including cytochrome b, NADH1, NADH2, NADH3, NADH4, NADH4L, NADH5, ATPase6, ATPase8, cytochrome c oxidase subunit 1, cytochrome c oxidase subunit 2, cytochrome c oxidase subunit 3 were analyzed in 30 head and neck squamous cell carcinoma (HNSCC) and the corresponding normal tissues using reverse transcriptase quantitative real time PCR. Pearson Chi-square test was used to determine the relationships between these expression levels and categorical parameters. Results: The expression levels of 12 mitochondrial mRNAs were observed in all 30 HNSCC patients with down-regulation, ranging from 43.3% to 76.7% and up-regulation, ranging from 10.0% to 36.7%. Furthermore, the number of cases with down-regulations in all 6 NADH and cytochrome b mRNA with TMN stages III and IV were significantly higher than that in stages I and II (p=0.049 and 0.007, respectively). Conclusion: Down-regulation of all mitochondrial NADH mRNA as well as mitochondrial cytochrome b mRNA was associated with high tumor stage among HNSCC patients. 2020-01-27T07:48:25Z 2020-01-27T07:48:25Z 2019-03-01 Article Archives of Oral Biology. Vol.99, (2019), 107-112 10.1016/j.archoralbio.2019.01.005 18791506 00039969 2-s2.0-85059900769 https://repository.li.mahidol.ac.th/handle/123456789/50241 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059900769&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Biochemistry, Genetics and Molecular Biology Dentistry Medicine |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Dentistry Medicine Pitsapaporn Wangpermtam Songsak Petmitr Phaibul Punyarit Boworn Klongnoi Sirima Sanguansin Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma |
description |
© 2019 Elsevier Ltd Objective: This study aimed to determine mitochondrial mRNA expression levels and the relationships between these expression levels and various adverse clinicopathological characteristics. Methods: The mRNA expression levels of all 12 genes encoded protein, located on the heavy-strand of mitochondrial DNA including cytochrome b, NADH1, NADH2, NADH3, NADH4, NADH4L, NADH5, ATPase6, ATPase8, cytochrome c oxidase subunit 1, cytochrome c oxidase subunit 2, cytochrome c oxidase subunit 3 were analyzed in 30 head and neck squamous cell carcinoma (HNSCC) and the corresponding normal tissues using reverse transcriptase quantitative real time PCR. Pearson Chi-square test was used to determine the relationships between these expression levels and categorical parameters. Results: The expression levels of 12 mitochondrial mRNAs were observed in all 30 HNSCC patients with down-regulation, ranging from 43.3% to 76.7% and up-regulation, ranging from 10.0% to 36.7%. Furthermore, the number of cases with down-regulations in all 6 NADH and cytochrome b mRNA with TMN stages III and IV were significantly higher than that in stages I and II (p=0.049 and 0.007, respectively). Conclusion: Down-regulation of all mitochondrial NADH mRNA as well as mitochondrial cytochrome b mRNA was associated with high tumor stage among HNSCC patients. |
author2 |
Army Institute of Pathology |
author_facet |
Army Institute of Pathology Pitsapaporn Wangpermtam Songsak Petmitr Phaibul Punyarit Boworn Klongnoi Sirima Sanguansin |
format |
Article |
author |
Pitsapaporn Wangpermtam Songsak Petmitr Phaibul Punyarit Boworn Klongnoi Sirima Sanguansin |
author_sort |
Pitsapaporn Wangpermtam |
title |
Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma |
title_short |
Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma |
title_full |
Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma |
title_fullStr |
Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma |
title_full_unstemmed |
Down-regulation of mitochondrial NADH and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma |
title_sort |
down-regulation of mitochondrial nadh and cytochrome b gene associated with high tumor stages in head and neck squamous cell carcinoma |
publishDate |
2020 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/50241 |
_version_ |
1763489902558707712 |