Flagellum Is Responsible for Promoting Effects of Viable Escherichia coli on Calcium Oxalate Crystallization, Crystal Growth, and Crystal Aggregation
© Copyright © 2019 Kanlaya, Naruepantawart and Thongboonkerd. Urease-producing bacteria (especially Proteus mirabilis) can cause infection kidney stone. However, recent studies have shown that intact viable non-urease-producing bacteria such as Escherichia coli might also promote calcium oxalate (Ca...
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th-mahidol.509942020-01-27T16:22:41Z Flagellum Is Responsible for Promoting Effects of Viable Escherichia coli on Calcium Oxalate Crystallization, Crystal Growth, and Crystal Aggregation Rattiyaporn Kanlaya Orapan Naruepantawart Visith Thongboonkerd Faculty of Medicine, Siriraj Hospital, Mahidol University Immunology and Microbiology Medicine © Copyright © 2019 Kanlaya, Naruepantawart and Thongboonkerd. Urease-producing bacteria (especially Proteus mirabilis) can cause infection kidney stone. However, recent studies have shown that intact viable non-urease-producing bacteria such as Escherichia coli might also promote calcium oxalate (CaOx) kidney stone formation but with unclear mechanism. We thus hypothesized that some relevant bacterial components might be responsible for such promoting effects of the intact viable E. coli. Flagella, capsule, lipopolysaccharide (LPS), and outer membrane vesicles (OMVs) were isolated/purified and their stone modulatory activities were evaluated using CaOx crystallization, crystal growth, and crystal aggregation assays. Among these, flagella had the most potent promoting effects on CaOx crystallization, crystal growth, and crystal aggregation. Validation was performed by deflagellation demonstrating that the deflagellated intact viable E. coli had markedly reduced CaOx crystal modulatory activities in all aspects (comparable to those of the negative controls). Similarly, neutralization of the isolated/purified flagella using a specific anti-flagellin antibody, not an isotype control, could abolish the promoting effects of flagella. These findings provide direct evidence indicating that flagellum is responsible for the promoting effects of the viable E. coli on CaOx crystallization, crystal growth and aggregation. 2020-01-27T08:51:49Z 2020-01-27T08:51:49Z 2019-11-05 Article Frontiers in Microbiology. Vol.10, (2019) 10.3389/fmicb.2019.02507 1664302X 2-s2.0-85075370404 https://repository.li.mahidol.ac.th/handle/123456789/50994 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85075370404&origin=inward |
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Immunology and Microbiology Medicine Rattiyaporn Kanlaya Orapan Naruepantawart Visith Thongboonkerd Flagellum Is Responsible for Promoting Effects of Viable Escherichia coli on Calcium Oxalate Crystallization, Crystal Growth, and Crystal Aggregation |
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© Copyright © 2019 Kanlaya, Naruepantawart and Thongboonkerd. Urease-producing bacteria (especially Proteus mirabilis) can cause infection kidney stone. However, recent studies have shown that intact viable non-urease-producing bacteria such as Escherichia coli might also promote calcium oxalate (CaOx) kidney stone formation but with unclear mechanism. We thus hypothesized that some relevant bacterial components might be responsible for such promoting effects of the intact viable E. coli. Flagella, capsule, lipopolysaccharide (LPS), and outer membrane vesicles (OMVs) were isolated/purified and their stone modulatory activities were evaluated using CaOx crystallization, crystal growth, and crystal aggregation assays. Among these, flagella had the most potent promoting effects on CaOx crystallization, crystal growth, and crystal aggregation. Validation was performed by deflagellation demonstrating that the deflagellated intact viable E. coli had markedly reduced CaOx crystal modulatory activities in all aspects (comparable to those of the negative controls). Similarly, neutralization of the isolated/purified flagella using a specific anti-flagellin antibody, not an isotype control, could abolish the promoting effects of flagella. These findings provide direct evidence indicating that flagellum is responsible for the promoting effects of the viable E. coli on CaOx crystallization, crystal growth and aggregation. |
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Faculty of Medicine, Siriraj Hospital, Mahidol University |
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Faculty of Medicine, Siriraj Hospital, Mahidol University Rattiyaporn Kanlaya Orapan Naruepantawart Visith Thongboonkerd |
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Article |
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Rattiyaporn Kanlaya Orapan Naruepantawart Visith Thongboonkerd |
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Rattiyaporn Kanlaya |
title |
Flagellum Is Responsible for Promoting Effects of Viable Escherichia coli on Calcium Oxalate Crystallization, Crystal Growth, and Crystal Aggregation |
title_short |
Flagellum Is Responsible for Promoting Effects of Viable Escherichia coli on Calcium Oxalate Crystallization, Crystal Growth, and Crystal Aggregation |
title_full |
Flagellum Is Responsible for Promoting Effects of Viable Escherichia coli on Calcium Oxalate Crystallization, Crystal Growth, and Crystal Aggregation |
title_fullStr |
Flagellum Is Responsible for Promoting Effects of Viable Escherichia coli on Calcium Oxalate Crystallization, Crystal Growth, and Crystal Aggregation |
title_full_unstemmed |
Flagellum Is Responsible for Promoting Effects of Viable Escherichia coli on Calcium Oxalate Crystallization, Crystal Growth, and Crystal Aggregation |
title_sort |
flagellum is responsible for promoting effects of viable escherichia coli on calcium oxalate crystallization, crystal growth, and crystal aggregation |
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2020 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/50994 |
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1763491229963649024 |