Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion
© 2019 The Author(s). Background: Plasmodium vivax malaria elimination can only be achieved by the deployment of 8-aminoquinolines (primaquine and tafenoquine) in combination with ACT to kill both blood and liver-stage parasites. However, primaquine and the other 8-aminoquinolines cause dose-depende...
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th-mahidol.511112020-01-27T17:12:27Z Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion Germana Bancone Didier Menard Nimol Khim Saorin Kim Lydie Canier Chea Nguong Koukeo Phommasone Mayfong Mayxay Sabine Dittrich Malavanh Vongsouvath Nadine Fievet Jean Yves Le Hesran Valerie Briand Sommay Keomany Paul N. Newton Gornpan Gorsawun Kaelan Tardy Cindy S. Chu Orpreeya Rattanapalroj Le Thanh Dong Huynh Hong Quang Nguyen Tam-Uyen Nguyen Thuy-Nhien Tran Tinh Hien Michael Kalnoky Francois Nosten Foundation for Innovative New Diagnostics, Switzerland Institut Pasteur du Cambodge Universite Paris Descartes UCL Mahidol University Nuffield Department of Clinical Medicine Institut Pasteur, Paris Institute of Malariology Parasitology and Entomology - Quy Nhon (IMPE-QN) Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU) Bureau of Vector Borne Diseases Institute of Malariology-Parasitology and Entomology (IMPE) National Center for Parasitology, Entomology and Malaria Control (CNM) PATH Salavan Provincial Hospital University of Health Sciences Immunology and Microbiology Medicine © 2019 The Author(s). Background: Plasmodium vivax malaria elimination can only be achieved by the deployment of 8-aminoquinolines (primaquine and tafenoquine) in combination with ACT to kill both blood and liver-stage parasites. However, primaquine and the other 8-aminoquinolines cause dose-dependent haemolysis in subjects with G6PD deficiency, an X-linked disorder of red blood cells that is very common in populations living in tropical and subtropical areas. In order to inform safer use of 8-aminoquinolines in the Greater Mekong Subregion, a multi-centre study was carried out to assess the prevalence of G6PD deficiency and to identify the main G6PD variants in samples collected in Cambodia, Lao PDR, Myanmar, Thailand and Vietnam. Methods: Blood samples were collected in the five countries during National Malaria Surveys or during Population Surveys. During Population Surveys samples were characterized for G6PD phenotype using the Fluorescent Spot Test. Samples were then genotyped for a panel of G6PD mutations. Results: G6PD deficiency was found to be common in the region with an overall mean prevalence of deficient or mutated hemizygous males of 14.0%, ranging from a mean 7.3% in Thailand, 8.1% in Lao PDR, 8.9% in Vietnam, 15.8% in Myanmar and 18.8% in Cambodia. Mahidol and Viangchan mutations were the most common and widespread variants found among the nine investigated. Conclusions: Owing to the high prevalence of G6PD deficiency in the Greater Mekong Subregion, strategies for vivax malaria elimination should include point-of-care G6PD testing (both qualitative and quantitative) to allow safe and wide treatment with 8-aminoquinolines. 2020-01-27T09:01:41Z 2020-01-27T09:01:41Z 2019-01-23 Article Malaria Journal. Vol.18, No.1 (2019) 10.1186/s12936-019-2652-y 14752875 2-s2.0-85060396457 https://repository.li.mahidol.ac.th/handle/123456789/51111 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85060396457&origin=inward |
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Immunology and Microbiology Medicine Germana Bancone Didier Menard Nimol Khim Saorin Kim Lydie Canier Chea Nguong Koukeo Phommasone Mayfong Mayxay Sabine Dittrich Malavanh Vongsouvath Nadine Fievet Jean Yves Le Hesran Valerie Briand Sommay Keomany Paul N. Newton Gornpan Gorsawun Kaelan Tardy Cindy S. Chu Orpreeya Rattanapalroj Le Thanh Dong Huynh Hong Quang Nguyen Tam-Uyen Nguyen Thuy-Nhien Tran Tinh Hien Michael Kalnoky Francois Nosten Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion |
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© 2019 The Author(s). Background: Plasmodium vivax malaria elimination can only be achieved by the deployment of 8-aminoquinolines (primaquine and tafenoquine) in combination with ACT to kill both blood and liver-stage parasites. However, primaquine and the other 8-aminoquinolines cause dose-dependent haemolysis in subjects with G6PD deficiency, an X-linked disorder of red blood cells that is very common in populations living in tropical and subtropical areas. In order to inform safer use of 8-aminoquinolines in the Greater Mekong Subregion, a multi-centre study was carried out to assess the prevalence of G6PD deficiency and to identify the main G6PD variants in samples collected in Cambodia, Lao PDR, Myanmar, Thailand and Vietnam. Methods: Blood samples were collected in the five countries during National Malaria Surveys or during Population Surveys. During Population Surveys samples were characterized for G6PD phenotype using the Fluorescent Spot Test. Samples were then genotyped for a panel of G6PD mutations. Results: G6PD deficiency was found to be common in the region with an overall mean prevalence of deficient or mutated hemizygous males of 14.0%, ranging from a mean 7.3% in Thailand, 8.1% in Lao PDR, 8.9% in Vietnam, 15.8% in Myanmar and 18.8% in Cambodia. Mahidol and Viangchan mutations were the most common and widespread variants found among the nine investigated. Conclusions: Owing to the high prevalence of G6PD deficiency in the Greater Mekong Subregion, strategies for vivax malaria elimination should include point-of-care G6PD testing (both qualitative and quantitative) to allow safe and wide treatment with 8-aminoquinolines. |
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Foundation for Innovative New Diagnostics, Switzerland |
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Foundation for Innovative New Diagnostics, Switzerland Germana Bancone Didier Menard Nimol Khim Saorin Kim Lydie Canier Chea Nguong Koukeo Phommasone Mayfong Mayxay Sabine Dittrich Malavanh Vongsouvath Nadine Fievet Jean Yves Le Hesran Valerie Briand Sommay Keomany Paul N. Newton Gornpan Gorsawun Kaelan Tardy Cindy S. Chu Orpreeya Rattanapalroj Le Thanh Dong Huynh Hong Quang Nguyen Tam-Uyen Nguyen Thuy-Nhien Tran Tinh Hien Michael Kalnoky Francois Nosten |
format |
Article |
author |
Germana Bancone Didier Menard Nimol Khim Saorin Kim Lydie Canier Chea Nguong Koukeo Phommasone Mayfong Mayxay Sabine Dittrich Malavanh Vongsouvath Nadine Fievet Jean Yves Le Hesran Valerie Briand Sommay Keomany Paul N. Newton Gornpan Gorsawun Kaelan Tardy Cindy S. Chu Orpreeya Rattanapalroj Le Thanh Dong Huynh Hong Quang Nguyen Tam-Uyen Nguyen Thuy-Nhien Tran Tinh Hien Michael Kalnoky Francois Nosten |
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Germana Bancone |
title |
Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion |
title_short |
Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion |
title_full |
Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion |
title_fullStr |
Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion |
title_full_unstemmed |
Molecular characterization and mapping of glucose-6-phosphate dehydrogenase (G6PD) mutations in the Greater Mekong Subregion |
title_sort |
molecular characterization and mapping of glucose-6-phosphate dehydrogenase (g6pd) mutations in the greater mekong subregion |
publishDate |
2020 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/51111 |
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1763495913646456832 |