Asymptomatic natural human infections with the simian malaria parasites plasmodium cynomolgi and plasmodium knowlesi
© The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. Background In Southeast Asia, Plasmodium knowlesi, a parasite of long-tailed macaques (Macaca fascicularis), is an important cause of human malaria. Plasmodium cynomolgi also commonly infects t...
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th-mahidol.518612020-01-27T17:05:39Z Asymptomatic natural human infections with the simian malaria parasites plasmodium cynomolgi and plasmodium knowlesi Mallika Imwong Wanassanan Madmanee Kanokon Suwannasin Chanon Kunasol Thomas J. Peto Rupam Tripura Lorenz Von Seidlein Chea Nguon Chan Davoeung Nicholas P.J. Day Arjen M. Dondorp Nicholas J. White Mahidol University Nuffield Department of Clinical Medicine Provincial Health Department National Center for Parasitology, Entomology and Malaria Control Medicine © The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. Background In Southeast Asia, Plasmodium knowlesi, a parasite of long-tailed macaques (Macaca fascicularis), is an important cause of human malaria. Plasmodium cynomolgi also commonly infects these monkeys, but only one naturally acquired symptomatic human case has been reported previously. Methods Malariometric studies involving 5422 subjects (aged 6 months to 65 years) were conducted in 23 villages in Pailin and Battambang, western Cambodia. Parasite detection and genotyping was conducted on blood samples, using high-volume quantitative PCR (uPCR). Results Asymptomatic malaria parasite infections were detected in 1361 of 14732 samples (9.2%). Asymptomatic infections with nonhuman primate malaria parasites were found in 21 individuals living close to forested areas; P. cynomolgi was found in 11, P. knowlesi was found in 8, and P. vivax and P. cynomolgi were both found in 2. Only 2 subjects were female, and 14 were men aged 20-40 years. Geometric mean parasite densities were 3604 parasites/mL in P. cynomolgi infections and 52488 parasites/mL in P. knowlesi infections. All P. cynomolgi isolates had wild-type dihydrofolate reductase genes, in contrast to the very high prevalence of mutations in the human malaria parasites. Asymptomatic reappearance of P. cynomolgi occurred in 2 subjects 3 months after the first infection. Conclusions Asymptomatic naturally acquired P. cynomolgi and P. knowlesi infections can both occur in humans. Clinical Trials Registration NCT01872702. 2020-01-27T10:05:39Z 2020-01-27T10:05:39Z 2019-02-15 Article Journal of Infectious Diseases. Vol.219, No.5 (2019), 695-702 10.1093/infdis/jiy519 15376613 00221899 2-s2.0-85056255746 https://repository.li.mahidol.ac.th/handle/123456789/51861 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85056255746&origin=inward |
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Medicine Mallika Imwong Wanassanan Madmanee Kanokon Suwannasin Chanon Kunasol Thomas J. Peto Rupam Tripura Lorenz Von Seidlein Chea Nguon Chan Davoeung Nicholas P.J. Day Arjen M. Dondorp Nicholas J. White Asymptomatic natural human infections with the simian malaria parasites plasmodium cynomolgi and plasmodium knowlesi |
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© The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. Background In Southeast Asia, Plasmodium knowlesi, a parasite of long-tailed macaques (Macaca fascicularis), is an important cause of human malaria. Plasmodium cynomolgi also commonly infects these monkeys, but only one naturally acquired symptomatic human case has been reported previously. Methods Malariometric studies involving 5422 subjects (aged 6 months to 65 years) were conducted in 23 villages in Pailin and Battambang, western Cambodia. Parasite detection and genotyping was conducted on blood samples, using high-volume quantitative PCR (uPCR). Results Asymptomatic malaria parasite infections were detected in 1361 of 14732 samples (9.2%). Asymptomatic infections with nonhuman primate malaria parasites were found in 21 individuals living close to forested areas; P. cynomolgi was found in 11, P. knowlesi was found in 8, and P. vivax and P. cynomolgi were both found in 2. Only 2 subjects were female, and 14 were men aged 20-40 years. Geometric mean parasite densities were 3604 parasites/mL in P. cynomolgi infections and 52488 parasites/mL in P. knowlesi infections. All P. cynomolgi isolates had wild-type dihydrofolate reductase genes, in contrast to the very high prevalence of mutations in the human malaria parasites. Asymptomatic reappearance of P. cynomolgi occurred in 2 subjects 3 months after the first infection. Conclusions Asymptomatic naturally acquired P. cynomolgi and P. knowlesi infections can both occur in humans. Clinical Trials Registration NCT01872702. |
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Mahidol University |
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Mahidol University Mallika Imwong Wanassanan Madmanee Kanokon Suwannasin Chanon Kunasol Thomas J. Peto Rupam Tripura Lorenz Von Seidlein Chea Nguon Chan Davoeung Nicholas P.J. Day Arjen M. Dondorp Nicholas J. White |
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Article |
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Mallika Imwong Wanassanan Madmanee Kanokon Suwannasin Chanon Kunasol Thomas J. Peto Rupam Tripura Lorenz Von Seidlein Chea Nguon Chan Davoeung Nicholas P.J. Day Arjen M. Dondorp Nicholas J. White |
author_sort |
Mallika Imwong |
title |
Asymptomatic natural human infections with the simian malaria parasites plasmodium cynomolgi and plasmodium knowlesi |
title_short |
Asymptomatic natural human infections with the simian malaria parasites plasmodium cynomolgi and plasmodium knowlesi |
title_full |
Asymptomatic natural human infections with the simian malaria parasites plasmodium cynomolgi and plasmodium knowlesi |
title_fullStr |
Asymptomatic natural human infections with the simian malaria parasites plasmodium cynomolgi and plasmodium knowlesi |
title_full_unstemmed |
Asymptomatic natural human infections with the simian malaria parasites plasmodium cynomolgi and plasmodium knowlesi |
title_sort |
asymptomatic natural human infections with the simian malaria parasites plasmodium cynomolgi and plasmodium knowlesi |
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2020 |
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https://repository.li.mahidol.ac.th/handle/123456789/51861 |
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1763487285590884352 |