Visual genotyping of thalassemia by using pyrrolidinyl peptide nucleic acid probes immobilized on carboxymethylcellulose-modified paper and enzyme-induced pigmentation

• Main Authors: Nuttapon Jirakittiwut, Thongperm Munkongdee, Kanet Wongravee, Orapan Sripichai, Suthat Fucharoen, Thanit Praneenararat, Tirayut Vilaivan
• Other Authors: Chulalongkorn University
• Format: Article
• Published: 2020
• Subjects: Chemistry
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/54516
• Institution: Mahidol University

© 2020, Springer-Verlag GmbH Austria, part of Springer Nature. A simple probe pair was designed for the detection of hemoglobin E (HbE) genotype, a single-point mutation that leads to abnormal red blood cells commonly found in South East Asia. The key to differentiation is the use of a conformationa...