Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3

Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3

© 2020 The Authors In mammals, there are a number of kinases, including serine/threonine-protein kinase LATS1, that act as a core kinase of the Hippo pathway and that negatively regulate the Hippo effector protein YAP and its paralog TAZ. Using CRISPR/Cas9 technology, we established a stable LATS1 k...

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Main Authors: Chanchao Lorthongpanich, Chuti Laowtammathron, Nittaya Jiamvoraphong, Pimonwan Srisook, Pimjai Chingsuwanrote, Phatchanat Klaihmon, Supaporn Waeteekul, Yaowalak U-pratya, Surapol Issaragrisil
Other Authors: Faculty of Medicine, Siriraj Hospital, Mahidol University
Format: Article
Published: 2020
Subjects:
Biochemistry, Genetics and Molecular Biology
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/57672
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spelling th-mahidol.576722020-08-25T16:01:00Z Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3 Chanchao Lorthongpanich Chuti Laowtammathron Nittaya Jiamvoraphong Pimonwan Srisook Pimjai Chingsuwanrote Phatchanat Klaihmon Supaporn Waeteekul Yaowalak U-pratya Surapol Issaragrisil Faculty of Medicine, Siriraj Hospital, Mahidol University Wattanosoth Hospital Biochemistry, Genetics and Molecular Biology © 2020 The Authors In mammals, there are a number of kinases, including serine/threonine-protein kinase LATS1, that act as a core kinase of the Hippo pathway and that negatively regulate the Hippo effector protein YAP and its paralog TAZ. Using CRISPR/Cas9 technology, we established a stable LATS1 knockdown (LATS1-KD) iPSC from the MUSIi012-A cell line. The LATS1-KD iPSC MUSIi012-A-3 that was developed maintained both the normal karyotype and the pluripotent phenotype, and retained the ability to differentiate into all three embryonic germ layers. 2020-08-25T09:01:00Z 2020-08-25T09:01:00Z 2020-10-01 Article Stem Cell Research. Vol.48, (2020) 10.1016/j.scr.2020.101950 18767753 18735061 2-s2.0-85089190010 https://repository.li.mahidol.ac.th/handle/123456789/57672 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85089190010&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Chanchao Lorthongpanich
Chuti Laowtammathron
Nittaya Jiamvoraphong
Pimonwan Srisook
Pimjai Chingsuwanrote
Phatchanat Klaihmon
Supaporn Waeteekul
Yaowalak U-pratya
Surapol Issaragrisil
Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3
description © 2020 The Authors In mammals, there are a number of kinases, including serine/threonine-protein kinase LATS1, that act as a core kinase of the Hippo pathway and that negatively regulate the Hippo effector protein YAP and its paralog TAZ. Using CRISPR/Cas9 technology, we established a stable LATS1 knockdown (LATS1-KD) iPSC from the MUSIi012-A cell line. The LATS1-KD iPSC MUSIi012-A-3 that was developed maintained both the normal karyotype and the pluripotent phenotype, and retained the ability to differentiate into all three embryonic germ layers.
author2 Faculty of Medicine, Siriraj Hospital, Mahidol University
author_facet Faculty of Medicine, Siriraj Hospital, Mahidol University
Chanchao Lorthongpanich
Chuti Laowtammathron
Nittaya Jiamvoraphong
Pimonwan Srisook
Pimjai Chingsuwanrote
Phatchanat Klaihmon
Supaporn Waeteekul
Yaowalak U-pratya
Surapol Issaragrisil
format Article
author Chanchao Lorthongpanich
Chuti Laowtammathron
Nittaya Jiamvoraphong
Pimonwan Srisook
Pimjai Chingsuwanrote
Phatchanat Klaihmon
Supaporn Waeteekul
Yaowalak U-pratya
Surapol Issaragrisil
author_sort Chanchao Lorthongpanich
title Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3
title_short Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3
title_full Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3
title_fullStr Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3
title_full_unstemmed Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3
title_sort generation of a serine/threonine-protein kinase lats1 gene-edited ipsc musii012-a-3
publishDate 2020
url https://repository.li.mahidol.ac.th/handle/123456789/57672
_version_ 1763492925479583744

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