Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer
© 2020 John Wiley & Sons Ltd Non-destructive sampling methods offer practical advantages to detection and monitoring of viral pathogens in economically important farmed fish and broodstock. Here, we investigated whether blood, mucus and fin can be used as non-lethal sample sources for detectio...
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th-mahidol.598382021-03-24T11:51:10Z Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer Onanong Charoenwai Saengchan Senapin Ha Thanh Dong Molruedee Sonthi Suan Sunandha Rajabhat University Mahidol University Thailand National Center for Genetic Engineering and Biotechnology Burapha University Agricultural and Biological Sciences Veterinary © 2020 John Wiley & Sons Ltd Non-destructive sampling methods offer practical advantages to detection and monitoring of viral pathogens in economically important farmed fish and broodstock. Here, we investigated whether blood, mucus and fin can be used as non-lethal sample sources for detection of scale drop disease virus (SDDV) in farmed Asian sea bass, Lates calcarifer. Detection of SDDV was performed in parallel from three non-destructive and seven destructive sample types, collected from both clinically sick fish and subclinical fish obtained from an affected farm. The results showed that SDDV was detectable in all 10 sample types with the percentage ranging from 20% to 100%. Blood was the best non-destructive sample source exhibited by the fact that it yielded 100% SDDV-positive tests from both sick (n = 12, 95% CI: 69.9–99.2) and clinically healthy fish (n = 4, 95% CI: 39.6%–97.4%) and is considered a “sterile” sample. This study also revealed concurrent infection of SDDV and two ectoparasites Lernanthropus sp. and Diplectanum sp., in all affected fish (n = 8, 95% CI: 46.7–99.3) during the disease outbreak. These ectoparasites also tested positive for SDDV by PCR, indicating that they were potential sample sources for PCR-based detection of SDDV and possibly other viruses infecting Asian sea bass. 2020-11-18T07:55:50Z 2020-11-18T07:55:50Z 2020-01-01 Article Journal of Fish Diseases. (2020) 10.1111/jfd.13290 13652761 01407775 2-s2.0-85094215766 https://repository.li.mahidol.ac.th/handle/123456789/59838 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85094215766&origin=inward |
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Agricultural and Biological Sciences Veterinary Onanong Charoenwai Saengchan Senapin Ha Thanh Dong Molruedee Sonthi Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer |
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© 2020 John Wiley & Sons Ltd Non-destructive sampling methods offer practical advantages to detection and monitoring of viral pathogens in economically important farmed fish and broodstock. Here, we investigated whether blood, mucus and fin can be used as non-lethal sample sources for detection of scale drop disease virus (SDDV) in farmed Asian sea bass, Lates calcarifer. Detection of SDDV was performed in parallel from three non-destructive and seven destructive sample types, collected from both clinically sick fish and subclinical fish obtained from an affected farm. The results showed that SDDV was detectable in all 10 sample types with the percentage ranging from 20% to 100%. Blood was the best non-destructive sample source exhibited by the fact that it yielded 100% SDDV-positive tests from both sick (n = 12, 95% CI: 69.9–99.2) and clinically healthy fish (n = 4, 95% CI: 39.6%–97.4%) and is considered a “sterile” sample. This study also revealed concurrent infection of SDDV and two ectoparasites Lernanthropus sp. and Diplectanum sp., in all affected fish (n = 8, 95% CI: 46.7–99.3) during the disease outbreak. These ectoparasites also tested positive for SDDV by PCR, indicating that they were potential sample sources for PCR-based detection of SDDV and possibly other viruses infecting Asian sea bass. |
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Suan Sunandha Rajabhat University |
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Suan Sunandha Rajabhat University Onanong Charoenwai Saengchan Senapin Ha Thanh Dong Molruedee Sonthi |
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Onanong Charoenwai Saengchan Senapin Ha Thanh Dong Molruedee Sonthi |
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Onanong Charoenwai |
title |
Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer |
title_short |
Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer |
title_full |
Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer |
title_fullStr |
Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer |
title_full_unstemmed |
Detection of scale drop disease virus from non-destructive samples and ectoparasites of Asian sea bass, Lates calcarifer |
title_sort |
detection of scale drop disease virus from non-destructive samples and ectoparasites of asian sea bass, lates calcarifer |
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2020 |
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https://repository.li.mahidol.ac.th/handle/123456789/59838 |
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1763494618514587648 |