Preferential modification of CyaA-hemolysin by CyaC-acyltransferase through the catalytic Ser³⁰-His³³ dyad in esterolysis of palmitoyl-donor substrate devoid of acyl carrier proteins

• Main Authors: Mattayaus Yentongchai, Niramon Thamwiriyasati, Chompounoot Imtong, Hui Chun Li, Chanan Angsuthanasombat
• Other Authors: Tzu Chi University
• Format: Article
• Published: 2020
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/59854
• Institution: Mahidol University

© 2020 Elsevier Inc. We previously demonstrated that the ~130-kDa CyaA-hemolysin domain (CyaA-Hly) from Bordetella pertussis co-expressed with CyaC-acyltransferase in Escherichia coli was acylated at Lys983 and thus activated its hemolytic activity. Here, attempts were made to provide greater insigh...