Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation

© 2020, Springer-Verlag GmbH Germany, part of Springer Nature. Large amounts of Morus alba L. (MA) roots are needed as the source of active stilbenes in the industrial production of traditional medicines and cosmeceuticals. A recent investigation demonstrated resveratrol and its derivatives to be pr...

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Main Authors: Chadathorn Inyai, Gorawit Yusakul, Jukrapun Komaikul, Tharita Kitisripanya, Kittisak Likhitwitayawuid, Boonchoo Sritularak, Waraporn Putalun
Other Authors: Chulalongkorn University
Format: Article
Published: 2020
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/59896
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spelling th-mahidol.598962020-11-18T15:36:31Z Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation Chadathorn Inyai Gorawit Yusakul Jukrapun Komaikul Tharita Kitisripanya Kittisak Likhitwitayawuid Boonchoo Sritularak Waraporn Putalun Chulalongkorn University Walailak University Khon Kaen University Mahidol University Biochemistry, Genetics and Molecular Biology Chemical Engineering © 2020, Springer-Verlag GmbH Germany, part of Springer Nature. Large amounts of Morus alba L. (MA) roots are needed as the source of active stilbenes in the industrial production of traditional medicines and cosmeceuticals. A recent investigation demonstrated resveratrol and its derivatives to be promising anti-COVID-19 agents. However, conventional cultivation of MA does not meet the demand for its stilbenes, and root quality usually varies between crops. This study established the in vitro non-GMO root culture of MA and optimized the root density, precursor feeding, and elicitors for stilbene productivity. A root culture with optimal inoculum density (3 g/flask of 30 mL medium) accumulated mulberroside A, oxyresveratrol, and resveratrol at 18.7 ± 1.00 mg/g, 136 ± 5.05 µg/g, and 41.6 ± 5.84 µg/g dry weight (DW), respectively. The feeding of L-tyrosine shortened the time required to reach the stilbene productive stage. Root cultures co-treated with 200 µM methyl jasmonate and 2 mg/mL yeast extract accumulated the highest contents of mulberroside A (30.3 ± 2.68 mg/g DW), oxyresveratrol (68.6 ± 3.53 µg/g DW), and resveratrol (10.2 ± 0.53 µg/g DW). In summary, root culture is a promising and sustainable source of stilbenes for the development of health products and agents for further investigation as potential anti-COVID-19 agents. 2020-11-18T08:24:55Z 2020-11-18T08:24:55Z 2020-01-01 Article Bioprocess and Biosystems Engineering. (2020) 10.1007/s00449-020-02474-7 16157605 16157591 2-s2.0-85095754493 https://repository.li.mahidol.ac.th/handle/123456789/59896 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85095754493&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemical Engineering
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Chadathorn Inyai
Gorawit Yusakul
Jukrapun Komaikul
Tharita Kitisripanya
Kittisak Likhitwitayawuid
Boonchoo Sritularak
Waraporn Putalun
Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation
description © 2020, Springer-Verlag GmbH Germany, part of Springer Nature. Large amounts of Morus alba L. (MA) roots are needed as the source of active stilbenes in the industrial production of traditional medicines and cosmeceuticals. A recent investigation demonstrated resveratrol and its derivatives to be promising anti-COVID-19 agents. However, conventional cultivation of MA does not meet the demand for its stilbenes, and root quality usually varies between crops. This study established the in vitro non-GMO root culture of MA and optimized the root density, precursor feeding, and elicitors for stilbene productivity. A root culture with optimal inoculum density (3 g/flask of 30 mL medium) accumulated mulberroside A, oxyresveratrol, and resveratrol at 18.7 ± 1.00 mg/g, 136 ± 5.05 µg/g, and 41.6 ± 5.84 µg/g dry weight (DW), respectively. The feeding of L-tyrosine shortened the time required to reach the stilbene productive stage. Root cultures co-treated with 200 µM methyl jasmonate and 2 mg/mL yeast extract accumulated the highest contents of mulberroside A (30.3 ± 2.68 mg/g DW), oxyresveratrol (68.6 ± 3.53 µg/g DW), and resveratrol (10.2 ± 0.53 µg/g DW). In summary, root culture is a promising and sustainable source of stilbenes for the development of health products and agents for further investigation as potential anti-COVID-19 agents.
author2 Chulalongkorn University
author_facet Chulalongkorn University
Chadathorn Inyai
Gorawit Yusakul
Jukrapun Komaikul
Tharita Kitisripanya
Kittisak Likhitwitayawuid
Boonchoo Sritularak
Waraporn Putalun
format Article
author Chadathorn Inyai
Gorawit Yusakul
Jukrapun Komaikul
Tharita Kitisripanya
Kittisak Likhitwitayawuid
Boonchoo Sritularak
Waraporn Putalun
author_sort Chadathorn Inyai
title Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation
title_short Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation
title_full Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation
title_fullStr Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation
title_full_unstemmed Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation
title_sort improvement of stilbene production by mulberry morus alba root culture via precursor feeding and co-elicitation
publishDate 2020
url https://repository.li.mahidol.ac.th/handle/123456789/59896
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