The modification of Hematoxylin staining method for plasmodium SPP in human peripheral blood smear
Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.205.
Saved in:
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Proceeding Poster |
| Language: | English |
| Published: |
2016
|
| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/63344 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Mahidol University |
| Language: | English |
| id |
th-mahidol.63344 |
|---|---|
| record_format |
dspace |
| spelling |
th-mahidol.633442021-08-30T22:33:39Z The modification of Hematoxylin staining method for plasmodium SPP in human peripheral blood smear Vasant Khachonsaksumet วสันต์ ขจรศักดิ์สุเมธ Yudthana Samung ยุทธนา สามัง Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology Mahidol University. Faculty of Tropical Medicine. Department of Medical Entomology Hematoxylin Malaria Modification Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.205. Malarial disease remains one of the major public health problems in the tropical and subtropical countries. Nowadays, the microscopic diagnosis of this disease is essential; human peripheral blood smear is stained with Wright staining or Field staining and examined under light microscope for the detection of the parasite. Although the treatment continuously has been improved; however, For the best result of treatment, malarial count in the blood smear is always used during the period of treatment. In all others forms of malaria, the diagnosis is confirmed by the demonstration of nuclei and cytoplasm in red blood cell in a smear. The organism are best revealed by Wright’s stain, the nuclei appear as red round or red ovoid structures and the cytoplasm was bluish-violet in the reddishviolet red blood cell. The identification of the blood stages of closely allied species of parasites presents unusual difficulties, because the morphology and behavior of the organisms are easily affected by minute changes of technique. The most satisfactory procedure is to compare the blood smears under identical condition, i.e. at the same time, in similar stages of the infection in a host of the same age and species and stained by precisely similar methods, indicating the brand of strain, and the diluents. Malarial parasite in blood smears can be stained with a variety of dyes. Some of the stains consist of a complex mixture of refine methylene blue and eosin that result the nuclei of the organism appear as red round while the cytoplasm was bluish-violet in the reddish-violet red blood cell. In some cases, there were few malarial parasite in the blood smear and the color of the reddish-violet red blood cell interferes the identification. We modified hematoxylin staining method for the staining of Plasmodium spp in human peripheral blood smear. In this modification method, the parasite took on a bluish stain while the red blood cell was colorless. 2016-02-05T07:30:24Z 2021-08-30T15:33:39Z 2016-02-05T07:30:24Z 2021-08-30T15:33:39Z 2004 Proceeding Poster https://repository.li.mahidol.ac.th/handle/123456789/63344 eng |
| institution |
Mahidol University |
| building |
Mahidol University Library |
| continent |
Asia |
| country |
Thailand Thailand |
| content_provider |
Mahidol University Library |
| collection |
Mahidol University Institutional Repository |
| language |
English |
| topic |
Hematoxylin Malaria Modification |
| spellingShingle |
Hematoxylin Malaria Modification Vasant Khachonsaksumet วสันต์ ขจรศักดิ์สุเมธ Yudthana Samung ยุทธนา สามัง The modification of Hematoxylin staining method for plasmodium SPP in human peripheral blood smear |
| description |
Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.205. |
| author2 |
Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology |
| author_facet |
Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology Vasant Khachonsaksumet วสันต์ ขจรศักดิ์สุเมธ Yudthana Samung ยุทธนา สามัง |
| format |
Proceeding Poster |
| author |
Vasant Khachonsaksumet วสันต์ ขจรศักดิ์สุเมธ Yudthana Samung ยุทธนา สามัง |
| author_sort |
Vasant Khachonsaksumet |
| title |
The modification of Hematoxylin staining method for plasmodium SPP in human peripheral blood smear |
| title_short |
The modification of Hematoxylin staining method for plasmodium SPP in human peripheral blood smear |
| title_full |
The modification of Hematoxylin staining method for plasmodium SPP in human peripheral blood smear |
| title_fullStr |
The modification of Hematoxylin staining method for plasmodium SPP in human peripheral blood smear |
| title_full_unstemmed |
The modification of Hematoxylin staining method for plasmodium SPP in human peripheral blood smear |
| title_sort |
modification of hematoxylin staining method for plasmodium spp in human peripheral blood smear |
| publishDate |
2016 |
| url |
https://repository.li.mahidol.ac.th/handle/123456789/63344 |
| _version_ |
1763493447291895808 |