Angiostrongyliasis: analysis of a chromatographically purified antigen of Angiostrongylus cantonensis adult worms by immunoblot
Joint International Tropical Medicine Meeting 2001: Century Pard Hotel, Bangkok, Thailand 8-10 August 2001: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2001. p.203
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2016
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th-mahidol.633462021-08-30T22:35:45Z Angiostrongyliasis: analysis of a chromatographically purified antigen of Angiostrongylus cantonensis adult worms by immunoblot Paron Dekumyoy พารณ ดีคำย้อย Prapan Piyasatittan Mayura Sinnawong Papchanee Shanaha Chalit Komalamisra ชลิต โกมลมิศร์ Supaporn Nuamtanong สุภาพร น่วมทะนง Siriporn Nuchfaong Kritsanna Janyapoon Mahidol University. Faculty of Tropical Medicine. Department of Helminthology Angiostrongyliasis Angiostrongylus cantonensis Helminths Joint International Tropical Medicine Meeting 2001: Century Pard Hotel, Bangkok, Thailand 8-10 August 2001: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2001. p.203 A crude extract of female and male adult worms of Angiostrongylus cantonesis was partially putified through Sephacryl S-200 gel chromatography. The fraction peak 2 had been previously evaluated to be the best functional antigen among four peaks with a sensitivity of 96.3% and a specificity of 96.53% by indirect ILISA. In the present study, the antigen peak 2 was determined for its antigenicity by immunoblot for searching a diagnostic band. The detection of lgG from human angiostrongyliasis was responsible for the antigen of 31 kDa. Not with negative controls and eighteen of other helminthic infections. Unfortunately, it was crossreactive with toxocariasis (1/2) and hydatidosis (1/2) sera, but only one case of hydatidosis is proven and officially reported in Thailand. No toxocariasis case has ever been reported, as well. The antigen was a glycoprotein analysed by peroxidase-labelled Concanvalin A and Coomassie brilliant blue staining. It is necessary to study the specific antigenicity of the 31 kDa antigen by a sophisticated technique and for it to be proven more with different kinds and numbers of other parasitic infections, including toxocariasis and hydatidosis 2016-01-15T06:56:12Z 2021-08-30T15:35:45Z 2016-01-15T06:56:12Z 2021-08-30T15:35:45Z 2016-01-15 2001 Proceeding Poster https://repository.li.mahidol.ac.th/handle/123456789/63346 eng Mahidol University |
| institution |
Mahidol University |
| building |
Mahidol University Library |
| continent |
Asia |
| country |
Thailand Thailand |
| content_provider |
Mahidol University Library |
| collection |
Mahidol University Institutional Repository |
| language |
English |
| topic |
Angiostrongyliasis Angiostrongylus cantonensis Helminths |
| spellingShingle |
Angiostrongyliasis Angiostrongylus cantonensis Helminths Paron Dekumyoy พารณ ดีคำย้อย Prapan Piyasatittan Mayura Sinnawong Papchanee Shanaha Chalit Komalamisra ชลิต โกมลมิศร์ Supaporn Nuamtanong สุภาพร น่วมทะนง Siriporn Nuchfaong Kritsanna Janyapoon Angiostrongyliasis: analysis of a chromatographically purified antigen of Angiostrongylus cantonensis adult worms by immunoblot |
| description |
Joint International Tropical Medicine Meeting 2001: Century Pard Hotel, Bangkok, Thailand 8-10 August 2001: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2001. p.203 |
| author2 |
Mahidol University. Faculty of Tropical Medicine. Department of Helminthology |
| author_facet |
Mahidol University. Faculty of Tropical Medicine. Department of Helminthology Paron Dekumyoy พารณ ดีคำย้อย Prapan Piyasatittan Mayura Sinnawong Papchanee Shanaha Chalit Komalamisra ชลิต โกมลมิศร์ Supaporn Nuamtanong สุภาพร น่วมทะนง Siriporn Nuchfaong Kritsanna Janyapoon |
| format |
Proceeding Poster |
| author |
Paron Dekumyoy พารณ ดีคำย้อย Prapan Piyasatittan Mayura Sinnawong Papchanee Shanaha Chalit Komalamisra ชลิต โกมลมิศร์ Supaporn Nuamtanong สุภาพร น่วมทะนง Siriporn Nuchfaong Kritsanna Janyapoon |
| author_sort |
Paron Dekumyoy |
| title |
Angiostrongyliasis: analysis of a chromatographically purified antigen of Angiostrongylus cantonensis adult worms by immunoblot |
| title_short |
Angiostrongyliasis: analysis of a chromatographically purified antigen of Angiostrongylus cantonensis adult worms by immunoblot |
| title_full |
Angiostrongyliasis: analysis of a chromatographically purified antigen of Angiostrongylus cantonensis adult worms by immunoblot |
| title_fullStr |
Angiostrongyliasis: analysis of a chromatographically purified antigen of Angiostrongylus cantonensis adult worms by immunoblot |
| title_full_unstemmed |
Angiostrongyliasis: analysis of a chromatographically purified antigen of Angiostrongylus cantonensis adult worms by immunoblot |
| title_sort |
angiostrongyliasis: analysis of a chromatographically purified antigen of angiostrongylus cantonensis adult worms by immunoblot |
| publishDate |
2016 |
| url |
https://repository.li.mahidol.ac.th/handle/123456789/63346 |
| _version_ |
1763488067965943808 |