Zebrafish U6 promoter driving short-hairpin RNA expression for PmRab7 knockdown to inhibit yellow head virus infection in shrimp hemocytes

• Main Authors: Wilasinee Thaiprasansup, Yongyut Pewkliang, Hoa Khanh Tran Kiem, Lalana Nuntakarn, Suparat Taengchaiyaphum, Vanvimon Saksmerprome, Sakol Panyim, Timothy W. Flegel, Kallaya Sritunyalucksana, Suparerk Borwornpinyo
• Other Authors: Mahidol University
• Format: Article
• Published: 2022
• Subjects: Agricultural and Biological Sciences
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/72974

RNA interference (RNAi) was investigated as potential antiviral strategy to mitigate losses in shrimp aquaculture. With this aim, an effective short-hairpin RNA (shRNA) expressed intracellularly from bacterial vectors incorporating eukaryotic promoters offers an alternative to an injected synthetic...