Detection of Norovirus Recombinant GII.2[P16] Strains in Oysters in Thailand
Human norovirus causes sporadic and epidemic acute gastroenteritis worldwide, and the predominant strains are genotype GII.4 variants. Recently, a novel GII.17[P17] and a recombinant GII.2[P16] strain have been reported as the causes of gastroenteritis outbreaks. Outbreaks of norovirus are frequentl...
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th-mahidol.730732022-08-04T11:28:59Z Detection of Norovirus Recombinant GII.2[P16] Strains in Oysters in Thailand Leera Kittigul Kannika Pombubpa Kitwadee Rupprom Jinthapha Thasiri Vajira Hospital Mahidol University Agricultural and Biological Sciences Environmental Science Immunology and Microbiology Medicine Human norovirus causes sporadic and epidemic acute gastroenteritis worldwide, and the predominant strains are genotype GII.4 variants. Recently, a novel GII.17[P17] and a recombinant GII.2[P16] strain have been reported as the causes of gastroenteritis outbreaks. Outbreaks of norovirus are frequently associated with foodborne illness. In this study, each of 75 oyster samples processed by a proteinase K extraction method and an adsorption-elution method were examined for noroviruses using RT-nested PCR with capsid primers. Thirteen (17.3%) samples processed by either method tested positive for norovirus genogroup II (GII). PCR amplicons were characterized by DNA sequencing and phylogenetic analysis as GII.2 (n = 6), GII.4 (n = 1), GII.17 (n = 3), and GII.unclassified (n = 3). Norovirus-positive samples were further amplified by semi-nested RT-PCR targeting the polymerase-capsid genes. One nucleotide sequence revealed GII.17[P17] Kawasaki strain. Five nucleotide sequences were identified as belonging to the recombinant GII.2[P16] strains by recombination analysis. The collected oyster samples were quantified for norovirus GII genome copy number by RT-quantitative PCR. Using the proteinase K method, GII was found in 13/75 (17.3%) of samples with a range of 8.83–1.85 × 104 genome copies/g of oyster. One sample (1/75, 1.3%) processed by the adsorption-elution method was positive for GII at 5.00 × 101 genome copies/g. These findings indicate the circulation of a new variant GII.17 Kawasaki strain and the recombinant GII.2[P16] in oyster samples corresponding to the circulating strains reported at a global scale during the same period of time. The detection of the recombinant strains in oysters emphasizes the need for continuing systematic surveillance for control and prevention of norovirus gastroenteritis. 2022-08-04T03:35:43Z 2022-08-04T03:35:43Z 2022-03-01 Article Food and Environmental Virology. Vol.14, No.1 (2022), 59-68 10.1007/s12560-022-09508-1 18670342 18670334 2-s2.0-85123492769 https://repository.li.mahidol.ac.th/handle/123456789/73073 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85123492769&origin=inward |
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Agricultural and Biological Sciences Environmental Science Immunology and Microbiology Medicine Leera Kittigul Kannika Pombubpa Kitwadee Rupprom Jinthapha Thasiri Detection of Norovirus Recombinant GII.2[P16] Strains in Oysters in Thailand |
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Human norovirus causes sporadic and epidemic acute gastroenteritis worldwide, and the predominant strains are genotype GII.4 variants. Recently, a novel GII.17[P17] and a recombinant GII.2[P16] strain have been reported as the causes of gastroenteritis outbreaks. Outbreaks of norovirus are frequently associated with foodborne illness. In this study, each of 75 oyster samples processed by a proteinase K extraction method and an adsorption-elution method were examined for noroviruses using RT-nested PCR with capsid primers. Thirteen (17.3%) samples processed by either method tested positive for norovirus genogroup II (GII). PCR amplicons were characterized by DNA sequencing and phylogenetic analysis as GII.2 (n = 6), GII.4 (n = 1), GII.17 (n = 3), and GII.unclassified (n = 3). Norovirus-positive samples were further amplified by semi-nested RT-PCR targeting the polymerase-capsid genes. One nucleotide sequence revealed GII.17[P17] Kawasaki strain. Five nucleotide sequences were identified as belonging to the recombinant GII.2[P16] strains by recombination analysis. The collected oyster samples were quantified for norovirus GII genome copy number by RT-quantitative PCR. Using the proteinase K method, GII was found in 13/75 (17.3%) of samples with a range of 8.83–1.85 × 104 genome copies/g of oyster. One sample (1/75, 1.3%) processed by the adsorption-elution method was positive for GII at 5.00 × 101 genome copies/g. These findings indicate the circulation of a new variant GII.17 Kawasaki strain and the recombinant GII.2[P16] in oyster samples corresponding to the circulating strains reported at a global scale during the same period of time. The detection of the recombinant strains in oysters emphasizes the need for continuing systematic surveillance for control and prevention of norovirus gastroenteritis. |
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Vajira Hospital |
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Vajira Hospital Leera Kittigul Kannika Pombubpa Kitwadee Rupprom Jinthapha Thasiri |
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Article |
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Leera Kittigul Kannika Pombubpa Kitwadee Rupprom Jinthapha Thasiri |
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Leera Kittigul |
title |
Detection of Norovirus Recombinant GII.2[P16] Strains in Oysters in Thailand |
title_short |
Detection of Norovirus Recombinant GII.2[P16] Strains in Oysters in Thailand |
title_full |
Detection of Norovirus Recombinant GII.2[P16] Strains in Oysters in Thailand |
title_fullStr |
Detection of Norovirus Recombinant GII.2[P16] Strains in Oysters in Thailand |
title_full_unstemmed |
Detection of Norovirus Recombinant GII.2[P16] Strains in Oysters in Thailand |
title_sort |
detection of norovirus recombinant gii.2[p16] strains in oysters in thailand |
publishDate |
2022 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/73073 |
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1763496533175566336 |