Micropropagation of Some Orchids and the Use of Cryopreservation

• Main Authors: Kanchit Thammasiri, Nipawan Jitsopakul, Sasikarn Prasongsom
• Other Authors: Rajamangala University of Technology Isan
• Format: Book Chapter
• Published: 2022
• Subjects: Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Chemistry; Pharmacology, Toxicology and Pharmaceutics
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/73147
• Institution: Mahidol University

Orchid micropropagation is an indispensable tool for seed and clonal propagation, especially for commercial purposes. It has been interestingly researched and used for enormous species and hybrids. In contrary, many wild orchid species are in endangered and extinction because of deforestation and natural disaster. Only outstanding horticultural characteristic orchids are cultivated. Therefore, orchid conservation is urgently needed by various means, such as living collection, seed and pollen storage, in vitro conservation, and cryopreservation for small aseptic materials, such as protocorms, protocorm-like bodies (PLBs), buds, root tips, meristem, callus, and cell suspension. In this review, orchid cryopreservation which is a long-term storage with genetic stability is focused for the development and successful use.