Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts

MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A...

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Main Authors:	Sukanya Chumchuen, Chonthicha Satirapod, Amornrat Tangprasittipap, Suradej Hongeng
Other Authors:	Faculty of Medicine Ramathibodi Hospital, Mahidol University
Format:	Article
Published:	2022
Subjects:	Biochemistry, Genetics and Molecular Biology
Online Access:	https://repository.li.mahidol.ac.th/handle/123456789/73381
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Institution:	Mahidol University

id	th-mahidol.73381
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spelling	th-mahidol.733812022-08-04T10:42:29Z Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts Sukanya Chumchuen Chonthicha Satirapod Amornrat Tangprasittipap Suradej Hongeng Faculty of Medicine Ramathibodi Hospital, Mahidol University Biochemistry, Genetics and Molecular Biology MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A cell line had embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a control to study disease mechanisms. 2022-08-04T03:42:29Z 2022-08-04T03:42:29Z 2022-04-01 Article Stem Cell Research. Vol.60, (2022) 10.1016/j.scr.2022.102675 18767753 18735061 2-s2.0-85123775729 https://repository.li.mahidol.ac.th/handle/123456789/73381 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85123775729&origin=inward
institution	Mahidol University
building	Mahidol University Library
continent	Asia
country	Thailand Thailand
content_provider	Mahidol University Library
collection	Mahidol University Institutional Repository
topic	Biochemistry, Genetics and Molecular Biology
spellingShingle	Biochemistry, Genetics and Molecular Biology Sukanya Chumchuen Chonthicha Satirapod Amornrat Tangprasittipap Suradej Hongeng Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts
description	MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A cell line had embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a control to study disease mechanisms.
author2	Faculty of Medicine Ramathibodi Hospital, Mahidol University
author_facet	Faculty of Medicine Ramathibodi Hospital, Mahidol University Sukanya Chumchuen Chonthicha Satirapod Amornrat Tangprasittipap Suradej Hongeng
format	Article
author	Sukanya Chumchuen Chonthicha Satirapod Amornrat Tangprasittipap Suradej Hongeng
author_sort	Sukanya Chumchuen
title	Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts
title_short	Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts
title_full	Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts
title_fullStr	Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts
title_full_unstemmed	Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts
title_sort	establishment of human induced pluripotent stem cell line mui028-a from normal fetal skin fibroblasts
publishDate	2022
url	https://repository.li.mahidol.ac.th/handle/123456789/73381
_version_	1763496794473365504

Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts

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