Performance Evaluation of BD FACSPresto™ Near-Patient CD4 Counter for Monitoring Antiretroviral Therapy in HIV-Infected Individuals in Primary Healthcare Clinics in Thailand
HIV viral load is more reliable tool for monitoring treatment throughout the course of HIV/AIDS, but the test may be expensive in resource-limited settings. Therefore, enumeration of CD4 T-lymphocyte count remains important in these settings. This study evaluated the performance of BDFACSPresto, a n...
Saved in:
Main Authors: | , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2022
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/73443 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.73443 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.734432022-08-04T10:43:50Z Performance Evaluation of BD FACSPresto™ Near-Patient CD4 Counter for Monitoring Antiretroviral Therapy in HIV-Infected Individuals in Primary Healthcare Clinics in Thailand Kasama Sukapirom Somrat Matchua Charin Thepthai Narinee Srimark Ladawan Khowawisetsut Kovit Pattanapanyasat Siriraj Hospital Chonburi Regional Hospital Biochemistry, Genetics and Molecular Biology HIV viral load is more reliable tool for monitoring treatment throughout the course of HIV/AIDS, but the test may be expensive in resource-limited settings. Therefore, enumeration of CD4 T-lymphocyte count remains important in these settings. This study evaluated the performance of BDFACSPresto, a near-patient CD4 counter planned to be used in primary healthcare clinics in Thailand. Results of percent, absolute CD4 count and hemoglobin (Hb) on the FACSPresto were compared with the TriTEST/TruCOUNT/BDFACSCalibur method and a Sysmex hematology ana-lyzer. Phase I of the study was performed in an ISO15189 laboratory. Both percentage and absolute values showed Passing–Bablok slopes within 0.98–1.06 and 0.97–1.13, mean Bland–Altman biases of +1.2% and +20.5 cells/µL, respectively. In phase II, venous and some capillary blood samples were analyzed in four primary healthcare clinics. The results showed good correlation between capillary and venous blood. For venous blood samples, regression lines showed slopes of 1.01–1.05 and 1.01– 1.07 for all percentage and absolute values. The overall mean biases were +0.9% and +17.0 cells/µL. For Hb, Passing–Bablok regression result gave slope within 1.01–1.07 and mean bias of −0.06 g/dL. Thus, CD4 enumeration in blood by the FACSPresto is reliable and can be performed to an identical standard at primary healthcare clinics. 2022-08-04T03:43:50Z 2022-08-04T03:43:50Z 2022-02-01 Article Diagnostics. Vol.12, No.2 (2022) 10.3390/diagnostics12020382 20754418 2-s2.0-85124075989 https://repository.li.mahidol.ac.th/handle/123456789/73443 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85124075989&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Biochemistry, Genetics and Molecular Biology |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Kasama Sukapirom Somrat Matchua Charin Thepthai Narinee Srimark Ladawan Khowawisetsut Kovit Pattanapanyasat Performance Evaluation of BD FACSPresto™ Near-Patient CD4 Counter for Monitoring Antiretroviral Therapy in HIV-Infected Individuals in Primary Healthcare Clinics in Thailand |
description |
HIV viral load is more reliable tool for monitoring treatment throughout the course of HIV/AIDS, but the test may be expensive in resource-limited settings. Therefore, enumeration of CD4 T-lymphocyte count remains important in these settings. This study evaluated the performance of BDFACSPresto, a near-patient CD4 counter planned to be used in primary healthcare clinics in Thailand. Results of percent, absolute CD4 count and hemoglobin (Hb) on the FACSPresto were compared with the TriTEST/TruCOUNT/BDFACSCalibur method and a Sysmex hematology ana-lyzer. Phase I of the study was performed in an ISO15189 laboratory. Both percentage and absolute values showed Passing–Bablok slopes within 0.98–1.06 and 0.97–1.13, mean Bland–Altman biases of +1.2% and +20.5 cells/µL, respectively. In phase II, venous and some capillary blood samples were analyzed in four primary healthcare clinics. The results showed good correlation between capillary and venous blood. For venous blood samples, regression lines showed slopes of 1.01–1.05 and 1.01– 1.07 for all percentage and absolute values. The overall mean biases were +0.9% and +17.0 cells/µL. For Hb, Passing–Bablok regression result gave slope within 1.01–1.07 and mean bias of −0.06 g/dL. Thus, CD4 enumeration in blood by the FACSPresto is reliable and can be performed to an identical standard at primary healthcare clinics. |
author2 |
Siriraj Hospital |
author_facet |
Siriraj Hospital Kasama Sukapirom Somrat Matchua Charin Thepthai Narinee Srimark Ladawan Khowawisetsut Kovit Pattanapanyasat |
format |
Article |
author |
Kasama Sukapirom Somrat Matchua Charin Thepthai Narinee Srimark Ladawan Khowawisetsut Kovit Pattanapanyasat |
author_sort |
Kasama Sukapirom |
title |
Performance Evaluation of BD FACSPresto™ Near-Patient CD4 Counter for Monitoring Antiretroviral Therapy in HIV-Infected Individuals in Primary Healthcare Clinics in Thailand |
title_short |
Performance Evaluation of BD FACSPresto™ Near-Patient CD4 Counter for Monitoring Antiretroviral Therapy in HIV-Infected Individuals in Primary Healthcare Clinics in Thailand |
title_full |
Performance Evaluation of BD FACSPresto™ Near-Patient CD4 Counter for Monitoring Antiretroviral Therapy in HIV-Infected Individuals in Primary Healthcare Clinics in Thailand |
title_fullStr |
Performance Evaluation of BD FACSPresto™ Near-Patient CD4 Counter for Monitoring Antiretroviral Therapy in HIV-Infected Individuals in Primary Healthcare Clinics in Thailand |
title_full_unstemmed |
Performance Evaluation of BD FACSPresto™ Near-Patient CD4 Counter for Monitoring Antiretroviral Therapy in HIV-Infected Individuals in Primary Healthcare Clinics in Thailand |
title_sort |
performance evaluation of bd facspresto™ near-patient cd4 counter for monitoring antiretroviral therapy in hiv-infected individuals in primary healthcare clinics in thailand |
publishDate |
2022 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/73443 |
_version_ |
1763489859163389952 |