Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells
Previous studies have shown that chemotherapeutic efficacy could be enhanced with targeted drug delivery. Various DNA origami nanostructures have been investigated as drug carriers. Here, we compared drug delivery functionalities of three similar DNA origami nanostructures, Disc, Donut, and Sphere,...
Saved in:
Main Authors: | , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2022
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/73715 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.73715 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.737152022-08-04T11:36:18Z Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells Anuttara Udomprasert Chanida Wootthichairangsan Ratchanee Duangrat Supattra Chaithongyot Yuwei Zhang Rachel Nixon Wenyan Liu Risheng Wang Mathurose Ponglikitmongkol Thaned Kangsamaksin Missouri University of Science and Technology Mahidol University Burapha University Chemistry Engineering Materials Science Medicine Previous studies have shown that chemotherapeutic efficacy could be enhanced with targeted drug delivery. Various DNA origami nanostructures have been investigated as drug carriers. Here, we compared drug delivery functionalities of three similar DNA origami nanostructures, Disc, Donut, and Sphere, that differ in structural dimension. Our results demonstrated that Donut was the most stable and exhibited the highest Dox-loading capacity. MUC1 aptamer modification in our nanostructures increased cellular uptake in MUC1-high MCF-7. Among the three nanostructures, unmodified Donut exerted the highest Dox cytotoxicity in MCF-7, and MUC1 aptamer modification did not further improve its effect, implicating that Dox delivery by Donut was efficient. However, all Dox-loaded nanostructures showed comparable cytotoxicity in MDA-MB-231 due to the innate sensitivity of this cell line to Dox. Our results successfully demonstrated that functional properties of DNA origami nanocarriers could be tuned by structural design, and three-dimensional Donut appeared to be the most efficient nanocarrier. 2022-08-04T03:52:36Z 2022-08-04T03:52:36Z 2022-01-01 Article ACS Applied Bio Materials. (2022) 10.1021/acsabm.2c00114 25766422 2-s2.0-85130204053 https://repository.li.mahidol.ac.th/handle/123456789/73715 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85130204053&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Chemistry Engineering Materials Science Medicine |
spellingShingle |
Chemistry Engineering Materials Science Medicine Anuttara Udomprasert Chanida Wootthichairangsan Ratchanee Duangrat Supattra Chaithongyot Yuwei Zhang Rachel Nixon Wenyan Liu Risheng Wang Mathurose Ponglikitmongkol Thaned Kangsamaksin Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells |
description |
Previous studies have shown that chemotherapeutic efficacy could be enhanced with targeted drug delivery. Various DNA origami nanostructures have been investigated as drug carriers. Here, we compared drug delivery functionalities of three similar DNA origami nanostructures, Disc, Donut, and Sphere, that differ in structural dimension. Our results demonstrated that Donut was the most stable and exhibited the highest Dox-loading capacity. MUC1 aptamer modification in our nanostructures increased cellular uptake in MUC1-high MCF-7. Among the three nanostructures, unmodified Donut exerted the highest Dox cytotoxicity in MCF-7, and MUC1 aptamer modification did not further improve its effect, implicating that Dox delivery by Donut was efficient. However, all Dox-loaded nanostructures showed comparable cytotoxicity in MDA-MB-231 due to the innate sensitivity of this cell line to Dox. Our results successfully demonstrated that functional properties of DNA origami nanocarriers could be tuned by structural design, and three-dimensional Donut appeared to be the most efficient nanocarrier. |
author2 |
Missouri University of Science and Technology |
author_facet |
Missouri University of Science and Technology Anuttara Udomprasert Chanida Wootthichairangsan Ratchanee Duangrat Supattra Chaithongyot Yuwei Zhang Rachel Nixon Wenyan Liu Risheng Wang Mathurose Ponglikitmongkol Thaned Kangsamaksin |
format |
Article |
author |
Anuttara Udomprasert Chanida Wootthichairangsan Ratchanee Duangrat Supattra Chaithongyot Yuwei Zhang Rachel Nixon Wenyan Liu Risheng Wang Mathurose Ponglikitmongkol Thaned Kangsamaksin |
author_sort |
Anuttara Udomprasert |
title |
Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells |
title_short |
Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells |
title_full |
Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells |
title_fullStr |
Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells |
title_full_unstemmed |
Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells |
title_sort |
enhanced functional properties of three dna origami nanostructures as doxorubicin carriers to breast cancer cells |
publishDate |
2022 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/73715 |
_version_ |
1763495022140850176 |