Shrimp protected from a virus by feed containing yeast with a surface-displayed viral binding protein

• Main Authors: Vorawit Ananphongmanee, Napathip Lertpreedakorn, Suparat Taengchaiyaphum, Theppanya Charoenrat, Kallaya Sritunyalucksana, Chuenchit Boonchird
• Other Authors: Mahidol University
• Format: Article
• Published: 2022
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/75886

Recombinant Pichia pastoris biomass surface-expressing the viral binding protein PmRab7 (YSD-PmRab7) was prepared by fed-batch, aerobic fermentation with methanol induction for 48 h. By cell based ELISA assay, immunofluorescence and flow cytometry, 45% of the YSD-PmRab7 cells were positive for PmRab...