Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept

Background: A platform for the utilization of the Chlorella sp. biomass and sugarcane leaves to produce multiple products (biorefinery concept) including hydrogen, methane, polyhydroxyalkanoates (PHAs), lipid, and soil supplement with the goal to achieve the zero waste generation (circular economy)...

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Main Authors: Napapat Sitthikitpanya, Sureewan Sittijunda, Sontaya Khamtib, Alissara Reungsang
Other Authors: Faculty of Environment and Resource Studies, Mahidol University
Format: Article
Published: 2022
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/75916
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spelling th-mahidol.759162022-08-04T15:45:53Z Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept Napapat Sitthikitpanya Sureewan Sittijunda Sontaya Khamtib Alissara Reungsang Faculty of Environment and Resource Studies, Mahidol University Khon Kaen University Academy of Science Agricultural Production Science Research and Development Division Biochemistry, Genetics and Molecular Biology Energy Environmental Science Immunology and Microbiology Background: A platform for the utilization of the Chlorella sp. biomass and sugarcane leaves to produce multiple products (biorefinery concept) including hydrogen, methane, polyhydroxyalkanoates (PHAs), lipid, and soil supplement with the goal to achieve the zero waste generation (circular economy) is demonstrated in this study. Microalgal biomass were hydrolyzed by mixed enzymes while sugarcane leaves were pretreated with alkali followed by enzyme. Hydrolysates were used to produce hydrogen and the hydrogenic effluent was used to produce multi-products. Solid residues at the end of hydrogen fermentation and the remaining acidified slurries from methane production were evaluated for the compost properties. Results: The maximum hydrogen yield of 207.65 mL-H2/g-volatile solid (VS)added was obtained from 0.92, 15.27, and 3.82 g-VS/L of Chlorella sp. biomass hydrolysate, sugarcane leaf hydrolysate, and anaerobic sludge, respectively. Hydrogenic effluent produced 321.1 mL/g-VS of methane yield, 2.01 g/L PHAs concentration, and 0.20 g/L of lipid concentration. Solid residues and the acidified slurries at the end of the hydrogen and methane production process were proved to have compost properties. Conclusion: Hydrogen production followed by methane, PHA and lipid productions is a successful integrated circular biorefinery platform to efficiently utilize the hydrolysates of Chlorella sp. biomass and sugarcane leaf. The potential use of the solid residues at the end of hydrogen fermentation and the remaining acidified slurries from methane production as soil supplements demonstrates the zero waste concept. The approach revealed in this study provides a foundation for the optimal use of feedstock, resulting in zero waste. Graphic Abstract: [Figure not available: see fulltext.] 2022-08-04T08:03:11Z 2022-08-04T08:03:11Z 2021-12-01 Article Biotechnology for Biofuels. Vol.14, No.1 (2021) 10.1186/s13068-021-02041-6 17546834 17546834 2-s2.0-85116359604 https://repository.li.mahidol.ac.th/handle/123456789/75916 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85116359604&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Energy
Environmental Science
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Energy
Environmental Science
Immunology and Microbiology
Napapat Sitthikitpanya
Sureewan Sittijunda
Sontaya Khamtib
Alissara Reungsang
Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept
description Background: A platform for the utilization of the Chlorella sp. biomass and sugarcane leaves to produce multiple products (biorefinery concept) including hydrogen, methane, polyhydroxyalkanoates (PHAs), lipid, and soil supplement with the goal to achieve the zero waste generation (circular economy) is demonstrated in this study. Microalgal biomass were hydrolyzed by mixed enzymes while sugarcane leaves were pretreated with alkali followed by enzyme. Hydrolysates were used to produce hydrogen and the hydrogenic effluent was used to produce multi-products. Solid residues at the end of hydrogen fermentation and the remaining acidified slurries from methane production were evaluated for the compost properties. Results: The maximum hydrogen yield of 207.65 mL-H2/g-volatile solid (VS)added was obtained from 0.92, 15.27, and 3.82 g-VS/L of Chlorella sp. biomass hydrolysate, sugarcane leaf hydrolysate, and anaerobic sludge, respectively. Hydrogenic effluent produced 321.1 mL/g-VS of methane yield, 2.01 g/L PHAs concentration, and 0.20 g/L of lipid concentration. Solid residues and the acidified slurries at the end of the hydrogen and methane production process were proved to have compost properties. Conclusion: Hydrogen production followed by methane, PHA and lipid productions is a successful integrated circular biorefinery platform to efficiently utilize the hydrolysates of Chlorella sp. biomass and sugarcane leaf. The potential use of the solid residues at the end of hydrogen fermentation and the remaining acidified slurries from methane production as soil supplements demonstrates the zero waste concept. The approach revealed in this study provides a foundation for the optimal use of feedstock, resulting in zero waste. Graphic Abstract: [Figure not available: see fulltext.]
author2 Faculty of Environment and Resource Studies, Mahidol University
author_facet Faculty of Environment and Resource Studies, Mahidol University
Napapat Sitthikitpanya
Sureewan Sittijunda
Sontaya Khamtib
Alissara Reungsang
format Article
author Napapat Sitthikitpanya
Sureewan Sittijunda
Sontaya Khamtib
Alissara Reungsang
author_sort Napapat Sitthikitpanya
title Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept
title_short Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept
title_full Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept
title_fullStr Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept
title_full_unstemmed Co-generation of biohydrogen and biochemicals from co-digestion of Chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept
title_sort co-generation of biohydrogen and biochemicals from co-digestion of chlorella sp. biomass hydrolysate with sugarcane leaf hydrolysate in an integrated circular biorefinery concept
publishDate 2022
url https://repository.li.mahidol.ac.th/handle/123456789/75916
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