A minipcr-duplex lateral flow dipstick platform for rapid and visual diagnosis of lymphatic filariae infection

Lymphatic filariasis (LF) is a neglected major tropical disease that is a leading cause of permanent and long-term disability worldwide. Significant progress made by the Global Programme to Eliminate Lymphatic Filariasis (GPELF) has led to a substantial decrease in the levels of infection. In this l...

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Main Authors: Achinya Phuakrod, Witsaroot Sripumkhai, Wutthinan Jeamsaksiri, Pattaraluck Pattamang, Sumat Loymek, Paul J. Brindley, Patsharaporn T. Sarasombath, Sirichit Wongkamchai
Other Authors: Siriraj Hospital
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Published: 2022
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/76003
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spelling th-mahidol.760032022-08-04T15:05:05Z A minipcr-duplex lateral flow dipstick platform for rapid and visual diagnosis of lymphatic filariae infection Achinya Phuakrod Witsaroot Sripumkhai Wutthinan Jeamsaksiri Pattaraluck Pattamang Sumat Loymek Paul J. Brindley Patsharaporn T. Sarasombath Sirichit Wongkamchai Siriraj Hospital The George Washington University School of Medicine and Health Sciences Thailand Ministry of Public Health Thailand National Electronics and Computer Technology Center Biochemistry, Genetics and Molecular Biology Lymphatic filariasis (LF) is a neglected major tropical disease that is a leading cause of permanent and long-term disability worldwide. Significant progress made by the Global Programme to Eliminate Lymphatic Filariasis (GPELF) has led to a substantial decrease in the levels of infection. In this limitation, DNA detection of lymphatic filariae could be useful due to it capable of detecting low level of the parasites. In the present study, we developed a diagnostic assay that combines a miniPCR with a duplex lateral flow dipstick (DLFD). The PCR primers were designed based on the HhaI and SspI repetitive noncoding DNA sequences of Brugia malayi and Wuchereria bancrofti, respectively. The limits of detection and crossreactivity of the assay were evaluated. In addition, blood samples were provided by Thais living in a brugian filariasis endemic area. The miniPCR-DLFD assay exhibited a detection limit of 2 and 4 mf per milliliter (mL) of blood for B. malayi as well as W. bancrofti, respectively, and crossamplification was not observed with 11 other parasites. The result obtained from the present study was in accordance with the thick blood smear staining for the known cases. Thus, a miniPCR-DLFD is an alternative tool for the diagnosis of LF in point-of-collection settings with a modest cost (~USD 5) per sample. 2022-08-04T08:05:05Z 2022-08-04T08:05:05Z 2021-10-01 Article Diagnostics. Vol.11, No.10 (2021) 10.3390/diagnostics11101855 20754418 2-s2.0-85117316472 https://repository.li.mahidol.ac.th/handle/123456789/76003 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85117316472&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Achinya Phuakrod
Witsaroot Sripumkhai
Wutthinan Jeamsaksiri
Pattaraluck Pattamang
Sumat Loymek
Paul J. Brindley
Patsharaporn T. Sarasombath
Sirichit Wongkamchai
A minipcr-duplex lateral flow dipstick platform for rapid and visual diagnosis of lymphatic filariae infection
description Lymphatic filariasis (LF) is a neglected major tropical disease that is a leading cause of permanent and long-term disability worldwide. Significant progress made by the Global Programme to Eliminate Lymphatic Filariasis (GPELF) has led to a substantial decrease in the levels of infection. In this limitation, DNA detection of lymphatic filariae could be useful due to it capable of detecting low level of the parasites. In the present study, we developed a diagnostic assay that combines a miniPCR with a duplex lateral flow dipstick (DLFD). The PCR primers were designed based on the HhaI and SspI repetitive noncoding DNA sequences of Brugia malayi and Wuchereria bancrofti, respectively. The limits of detection and crossreactivity of the assay were evaluated. In addition, blood samples were provided by Thais living in a brugian filariasis endemic area. The miniPCR-DLFD assay exhibited a detection limit of 2 and 4 mf per milliliter (mL) of blood for B. malayi as well as W. bancrofti, respectively, and crossamplification was not observed with 11 other parasites. The result obtained from the present study was in accordance with the thick blood smear staining for the known cases. Thus, a miniPCR-DLFD is an alternative tool for the diagnosis of LF in point-of-collection settings with a modest cost (~USD 5) per sample.
author2 Siriraj Hospital
author_facet Siriraj Hospital
Achinya Phuakrod
Witsaroot Sripumkhai
Wutthinan Jeamsaksiri
Pattaraluck Pattamang
Sumat Loymek
Paul J. Brindley
Patsharaporn T. Sarasombath
Sirichit Wongkamchai
format Article
author Achinya Phuakrod
Witsaroot Sripumkhai
Wutthinan Jeamsaksiri
Pattaraluck Pattamang
Sumat Loymek
Paul J. Brindley
Patsharaporn T. Sarasombath
Sirichit Wongkamchai
author_sort Achinya Phuakrod
title A minipcr-duplex lateral flow dipstick platform for rapid and visual diagnosis of lymphatic filariae infection
title_short A minipcr-duplex lateral flow dipstick platform for rapid and visual diagnosis of lymphatic filariae infection
title_full A minipcr-duplex lateral flow dipstick platform for rapid and visual diagnosis of lymphatic filariae infection
title_fullStr A minipcr-duplex lateral flow dipstick platform for rapid and visual diagnosis of lymphatic filariae infection
title_full_unstemmed A minipcr-duplex lateral flow dipstick platform for rapid and visual diagnosis of lymphatic filariae infection
title_sort minipcr-duplex lateral flow dipstick platform for rapid and visual diagnosis of lymphatic filariae infection
publishDate 2022
url https://repository.li.mahidol.ac.th/handle/123456789/76003
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