Application of one-step reverse transcription droplet digital pcr for dengue virus detection and quantification in clinical specimens

• Main Authors: Dumrong Mairiang, Adisak Songjaeng, Prachya Hansuealueang, Yuwares Malila, Paphavee Lertsethtakarn, Sasikorn Silapong, Yongyuth Poolpanichupatam, Chonticha Klungthong, Kwanrutai Chin-Inmanu, Somchai Thiemmeca, Nattaya Tangthawornchaikul, Kanokwan Sriraksa, Wannee Limpitikul, Sirijitt Vasanawathana, Damon W. Ellison, Prida Malasit, Prapat Suriyaphol, Panisadee Avirutnan
• Other Authors: Siriraj Hospital
• Format: Article
• Published: 2022
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/76216
• Institution: Mahidol University

Detection and quantification of viruses in laboratory and clinical samples are standard assays in dengue virus (DENV) studies. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) is considered to be the standard for DENV detection and quantification due to its high sensitivity...