Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography

Pueraria candollei is a phytoestrogen-rich herb used to treat estrogen deficiency disorders; however, quality control of P. candollei-related health products is required for consistency of clinical outcomes. Estrogenically active (+)-7-O-methylisomiroestrol could be a potential chemical marker that...

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Main Authors: Wipawee Juengsanguanpornsuk, Gorawit Yusakul, Tharita Kitisripanya, Supaluk Krittanai, Thaweesak Juengwatanatrakul, Seiichi Sakamoto, Waraporn Putalun
Other Authors: Ubon Ratchathani University
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Published: 2022
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/76307
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spelling th-mahidol.763072022-08-04T18:23:23Z Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography Wipawee Juengsanguanpornsuk Gorawit Yusakul Tharita Kitisripanya Supaluk Krittanai Thaweesak Juengwatanatrakul Seiichi Sakamoto Waraporn Putalun Ubon Ratchathani University Walailak University Khon Kaen University Mahidol University Kyushu University Biochemistry, Genetics and Molecular Biology Chemistry Pharmacology, Toxicology and Pharmaceutics Pueraria candollei is a phytoestrogen-rich herb used to treat estrogen deficiency disorders; however, quality control of P. candollei-related health products is required for consistency of clinical outcomes. Estrogenically active (+)-7-O-methylisomiroestrol could be a potential chemical marker that facilitates the prediction of the overall estrogenic activity of P. candollei. The analytical performance of ELISA using newly produced monoclonal antibodies against methylisomiroestrol was compared with HPLC analysis. The developed indirect competitive ELISA (icELISA) was highly sensitive to methylisomiroestrol for detection, with an LOQ of 2.9 ng/mL, whereas the LOQ was 1.15 μg/mL by HPLC. The results from method validation indicated acceptable precision (1.71–6.37 % and 0.13–2.40 %) and accuracy (99.23–102.54 % and 96.84–101.88 %) of the methylisomiroestrol analysis using icELISA and HPLC. These methods were effectively applied for the determination of the methylisomiroestrol content in P. candollei samples. Apart from the plant tubers, the stem was observed as a source of methylisomiroestrol. The developed ELISA was more effective than HPLC in detecting a small quantity of methylisomiroestrol in the plant samples [0.23 × 10−3% (w/w) to 0.628 × 10−3% (w/w) dry weight]. Therefore, the ELISA could be a useful tool for the standardization of P. candollei, which is the crucial step to improve the quality of plant-derived products. 2022-08-04T08:12:53Z 2022-08-04T08:12:53Z 2021-01-05 Article Journal of Pharmaceutical and Biomedical Analysis. Vol.192, (2021) 10.1016/j.jpba.2020.113674 1873264X 07317085 2-s2.0-85093694414 https://repository.li.mahidol.ac.th/handle/123456789/76307 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85093694414&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Pharmacology, Toxicology and Pharmaceutics
Wipawee Juengsanguanpornsuk
Gorawit Yusakul
Tharita Kitisripanya
Supaluk Krittanai
Thaweesak Juengwatanatrakul
Seiichi Sakamoto
Waraporn Putalun
Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography
description Pueraria candollei is a phytoestrogen-rich herb used to treat estrogen deficiency disorders; however, quality control of P. candollei-related health products is required for consistency of clinical outcomes. Estrogenically active (+)-7-O-methylisomiroestrol could be a potential chemical marker that facilitates the prediction of the overall estrogenic activity of P. candollei. The analytical performance of ELISA using newly produced monoclonal antibodies against methylisomiroestrol was compared with HPLC analysis. The developed indirect competitive ELISA (icELISA) was highly sensitive to methylisomiroestrol for detection, with an LOQ of 2.9 ng/mL, whereas the LOQ was 1.15 μg/mL by HPLC. The results from method validation indicated acceptable precision (1.71–6.37 % and 0.13–2.40 %) and accuracy (99.23–102.54 % and 96.84–101.88 %) of the methylisomiroestrol analysis using icELISA and HPLC. These methods were effectively applied for the determination of the methylisomiroestrol content in P. candollei samples. Apart from the plant tubers, the stem was observed as a source of methylisomiroestrol. The developed ELISA was more effective than HPLC in detecting a small quantity of methylisomiroestrol in the plant samples [0.23 × 10−3% (w/w) to 0.628 × 10−3% (w/w) dry weight]. Therefore, the ELISA could be a useful tool for the standardization of P. candollei, which is the crucial step to improve the quality of plant-derived products.
author2 Ubon Ratchathani University
author_facet Ubon Ratchathani University
Wipawee Juengsanguanpornsuk
Gorawit Yusakul
Tharita Kitisripanya
Supaluk Krittanai
Thaweesak Juengwatanatrakul
Seiichi Sakamoto
Waraporn Putalun
format Article
author Wipawee Juengsanguanpornsuk
Gorawit Yusakul
Tharita Kitisripanya
Supaluk Krittanai
Thaweesak Juengwatanatrakul
Seiichi Sakamoto
Waraporn Putalun
author_sort Wipawee Juengsanguanpornsuk
title Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography
title_short Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography
title_full Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography
title_fullStr Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography
title_full_unstemmed Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography
title_sort quantification of methylisomiroestrol, a phytoestrogen of pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography
publishDate 2022
url https://repository.li.mahidol.ac.th/handle/123456789/76307
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