Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa
A special-care neonatal unit from a large public hospital in Malawi was noted as having more frequent, difficult-to-treat infec-tions, and a suspected outbreak of multi-drug-resistant Klebsiella pneumoniae was investigated using genomic characterisa-tion. All K. pneumoniae bloodstream infections (BS...
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th-mahidol.763172022-08-04T18:03:53Z Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa Jennifer Cornick Patrick Musicha Chikondi Peno Ezgi Seager Pui Ying Iroh Tam Sithembile Bilima Aisleen Bennett Neil Kennedy Nicholas Feasey Eva Heinz Amy K. Cain Malawi-Liverpool-Wellcome Trust Clinical Research Programme University of Malawi College of Medicine Queen's University Belfast St George’s, University of London Heartlands Hospital Liverpool School of Tropical Medicine University of Liverpool Macquarie University Mahidol University University of Edinburgh, College of Medicine and Veterinary Medicine Nuffield Department of Medicine Wellcome Sanger Institute Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine A special-care neonatal unit from a large public hospital in Malawi was noted as having more frequent, difficult-to-treat infec-tions, and a suspected outbreak of multi-drug-resistant Klebsiella pneumoniae was investigated using genomic characterisa-tion. All K. pneumoniae bloodstream infections (BSIs) from patients in the neonatal ward (n=62), and a subset of K. pneumoniae BSI isolates (n=38) from other paediatric wards in the hospital, collected over a 4 year period were studied. After whole genome sequencing, the strain sequence types (STs), plasmid types, virulence and resistance genes were identified. One ST340 clone, part of clonal complex 258 (CC258) and an ST that drives hospital outbreaks worldwide, harbouring numerous resistance genes and plasmids, was implicated as the likely cause of the outbreak. This study contributes molecular information necessary for tracking and characterizing this important hospital pathogen in sub-Saharan Africa. 2022-08-04T08:13:06Z 2022-08-04T08:13:06Z 2021-01-01 Article Microbial Genomics. Vol.7, No.11 (2021) 10.1099/mgen.0.000703 20575858 2-s2.0-85121993914 https://repository.li.mahidol.ac.th/handle/123456789/76317 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85121993914&origin=inward |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine Jennifer Cornick Patrick Musicha Chikondi Peno Ezgi Seager Pui Ying Iroh Tam Sithembile Bilima Aisleen Bennett Neil Kennedy Nicholas Feasey Eva Heinz Amy K. Cain Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa |
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A special-care neonatal unit from a large public hospital in Malawi was noted as having more frequent, difficult-to-treat infec-tions, and a suspected outbreak of multi-drug-resistant Klebsiella pneumoniae was investigated using genomic characterisa-tion. All K. pneumoniae bloodstream infections (BSIs) from patients in the neonatal ward (n=62), and a subset of K. pneumoniae BSI isolates (n=38) from other paediatric wards in the hospital, collected over a 4 year period were studied. After whole genome sequencing, the strain sequence types (STs), plasmid types, virulence and resistance genes were identified. One ST340 clone, part of clonal complex 258 (CC258) and an ST that drives hospital outbreaks worldwide, harbouring numerous resistance genes and plasmids, was implicated as the likely cause of the outbreak. This study contributes molecular information necessary for tracking and characterizing this important hospital pathogen in sub-Saharan Africa. |
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Malawi-Liverpool-Wellcome Trust Clinical Research Programme |
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Malawi-Liverpool-Wellcome Trust Clinical Research Programme Jennifer Cornick Patrick Musicha Chikondi Peno Ezgi Seager Pui Ying Iroh Tam Sithembile Bilima Aisleen Bennett Neil Kennedy Nicholas Feasey Eva Heinz Amy K. Cain |
format |
Article |
author |
Jennifer Cornick Patrick Musicha Chikondi Peno Ezgi Seager Pui Ying Iroh Tam Sithembile Bilima Aisleen Bennett Neil Kennedy Nicholas Feasey Eva Heinz Amy K. Cain |
author_sort |
Jennifer Cornick |
title |
Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa |
title_short |
Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa |
title_full |
Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa |
title_fullStr |
Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa |
title_full_unstemmed |
Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa |
title_sort |
genomic investigation of a suspected klebsiella pneumoniae outbreak in a neonatal care unit in sub-saharan africa |
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2022 |
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https://repository.li.mahidol.ac.th/handle/123456789/76317 |
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1763488699364933632 |