Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine
The arsenic speciation, the abundance of arsenite-oxidizing bacteria, and microbial community structures in the groundwater, surface water, and soil from a gold mining area were explored using the PHREEQC model, cloning-ddPCR of the aioA gene, and high-throughput sequencing of the 16S rRNA gene, res...
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th-mahidol.770742022-08-04T15:43:08Z Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine Prinpida Sonthiphand Supeerapat Kraidech Saowarod Polart Srilert Chotpantarat Kanthida Kusonmano Pichahpuk Uthaipaisanwong Chalida Rangsiwutisak Ekawan Luepromchai Chulalongkorn University Mahidol University King Mongkut's University of Technology Thonburi Environmental Science The arsenic speciation, the abundance of arsenite-oxidizing bacteria, and microbial community structures in the groundwater, surface water, and soil from a gold mining area were explored using the PHREEQC model, cloning-ddPCR of the aioA gene, and high-throughput sequencing of the 16S rRNA gene, respectively. The analysis of the aioA gene showed that arsenite-oxidizing bacteria retrieved from groundwater, surface water, and soil were associated with Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. In groundwaters from the mining area, there were relatively high ratios of aioA/total 16S rRNA gene copies and the dominance of As5+, which suggested the presence and activity of arsenite-oxidizing bacteria. Metagenomic analysis revealed that the majority of the soil and surface water microbiomes were Proteobacteria, Actinobacteria, Bacteroidetes, and Chloroflexi, whereas the groundwater microbiomes were dominated exclusively by Betaproteobacteria and Alphaproteobacteria. Geochemical factors influencing the microbial structure in the groundwater were As, residence time, and groundwater flowrate, while those showing a positive correlation to the microbial structure in the surface water were TOC, ORP, and DO. This study provides insights into the groundwater, surface water, and soil microbiomes from a gold mine and expands the current understanding of the diversity and abundance of arsenite-oxidizing bacteria, playing a vital role in global As cycling. 2022-08-04T08:43:08Z 2022-08-04T08:43:08Z 2021-01-01 Article Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering. Vol.56, No.7 (2021), 769-785 10.1080/10934529.2021.1927421 15324117 10934529 2-s2.0-85106517702 https://repository.li.mahidol.ac.th/handle/123456789/77074 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85106517702&origin=inward |
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Environmental Science Prinpida Sonthiphand Supeerapat Kraidech Saowarod Polart Srilert Chotpantarat Kanthida Kusonmano Pichahpuk Uthaipaisanwong Chalida Rangsiwutisak Ekawan Luepromchai Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine |
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The arsenic speciation, the abundance of arsenite-oxidizing bacteria, and microbial community structures in the groundwater, surface water, and soil from a gold mining area were explored using the PHREEQC model, cloning-ddPCR of the aioA gene, and high-throughput sequencing of the 16S rRNA gene, respectively. The analysis of the aioA gene showed that arsenite-oxidizing bacteria retrieved from groundwater, surface water, and soil were associated with Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. In groundwaters from the mining area, there were relatively high ratios of aioA/total 16S rRNA gene copies and the dominance of As5+, which suggested the presence and activity of arsenite-oxidizing bacteria. Metagenomic analysis revealed that the majority of the soil and surface water microbiomes were Proteobacteria, Actinobacteria, Bacteroidetes, and Chloroflexi, whereas the groundwater microbiomes were dominated exclusively by Betaproteobacteria and Alphaproteobacteria. Geochemical factors influencing the microbial structure in the groundwater were As, residence time, and groundwater flowrate, while those showing a positive correlation to the microbial structure in the surface water were TOC, ORP, and DO. This study provides insights into the groundwater, surface water, and soil microbiomes from a gold mine and expands the current understanding of the diversity and abundance of arsenite-oxidizing bacteria, playing a vital role in global As cycling. |
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Chulalongkorn University |
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Chulalongkorn University Prinpida Sonthiphand Supeerapat Kraidech Saowarod Polart Srilert Chotpantarat Kanthida Kusonmano Pichahpuk Uthaipaisanwong Chalida Rangsiwutisak Ekawan Luepromchai |
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Prinpida Sonthiphand Supeerapat Kraidech Saowarod Polart Srilert Chotpantarat Kanthida Kusonmano Pichahpuk Uthaipaisanwong Chalida Rangsiwutisak Ekawan Luepromchai |
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Prinpida Sonthiphand |
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Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine |
title_short |
Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine |
title_full |
Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine |
title_fullStr |
Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine |
title_full_unstemmed |
Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine |
title_sort |
arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine |
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2022 |
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https://repository.li.mahidol.ac.th/handle/123456789/77074 |
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