Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates
Background: Plasmodium vivax transmission-blocking vaccines (TBVs) are receiving increasing attention. Based on excellent transmission-blocking activities of the PbPH (PBANKA_0417200) and PbSOP26 (PBANKA_1457700) antigens in Plasmodium berghei, their orthologs in P. vivax, PVX_098655 (PvPH) and PVX_...
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th-mahidol.771552022-08-04T16:02:44Z Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates Yongzhe Zhang Fei Liu Yan Zhao Fan Yang Jie Bai Xitong Jia Wanlapa Roobsoong Jetsumon Sattabongkot Liwang Cui Yaming Cao Enjie Luo Meilian Wang Faculty of Tropical Medicine, Mahidol University Morsani College of Medicine China Medical University Shenyang Immunology and Microbiology Medicine Background: Plasmodium vivax transmission-blocking vaccines (TBVs) are receiving increasing attention. Based on excellent transmission-blocking activities of the PbPH (PBANKA_0417200) and PbSOP26 (PBANKA_1457700) antigens in Plasmodium berghei, their orthologs in P. vivax, PVX_098655 (PvPH) and PVX_101120 (PvSOP26), were selected for the evaluation of their potential as TBVs. Methods: Fragments of PvPH (amino acids 22–304) and PvSOP26 (amino acids 30–272) were expressed in the yeast expression system. The recombinant proteins were used to immunize mice to obtain antisera. The transmission-reducing activities of these antisera were evaluated using the direct membrane feeding assay (DMFA) using Anopheles dirus mosquitoes and P. vivax clinical isolates. Results: The recombinant proteins PvPH and PvSOP26 induced robust antibody responses in mice. The DMFA showed that the anti-PvSOP26 sera significantly reduced oocyst densities by 92.0 and 84.1% in two parasite isolates, respectively, whereas the anti-PvPH sera did not show evident transmission-reducing activity. The variation in the DMFA results was unlikely due to the genetic polymorphisms of the two genes since their respective sequences were identical in the clinical P. vivax isolates. Conclusion: PvSOP26 could be a promising TBV candidate for P. vivax, which warrants further evaluation. Graphical Abstract: [Figure not available: see fulltext.] 2022-08-04T08:46:06Z 2022-08-04T08:46:06Z 2021-12-01 Article Parasites and Vectors. Vol.14, No.1 (2021) 10.1186/s13071-021-04909-w 17563305 2-s2.0-85112726658 https://repository.li.mahidol.ac.th/handle/123456789/77155 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85112726658&origin=inward |
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Immunology and Microbiology Medicine Yongzhe Zhang Fei Liu Yan Zhao Fan Yang Jie Bai Xitong Jia Wanlapa Roobsoong Jetsumon Sattabongkot Liwang Cui Yaming Cao Enjie Luo Meilian Wang Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates |
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Background: Plasmodium vivax transmission-blocking vaccines (TBVs) are receiving increasing attention. Based on excellent transmission-blocking activities of the PbPH (PBANKA_0417200) and PbSOP26 (PBANKA_1457700) antigens in Plasmodium berghei, their orthologs in P. vivax, PVX_098655 (PvPH) and PVX_101120 (PvSOP26), were selected for the evaluation of their potential as TBVs. Methods: Fragments of PvPH (amino acids 22–304) and PvSOP26 (amino acids 30–272) were expressed in the yeast expression system. The recombinant proteins were used to immunize mice to obtain antisera. The transmission-reducing activities of these antisera were evaluated using the direct membrane feeding assay (DMFA) using Anopheles dirus mosquitoes and P. vivax clinical isolates. Results: The recombinant proteins PvPH and PvSOP26 induced robust antibody responses in mice. The DMFA showed that the anti-PvSOP26 sera significantly reduced oocyst densities by 92.0 and 84.1% in two parasite isolates, respectively, whereas the anti-PvPH sera did not show evident transmission-reducing activity. The variation in the DMFA results was unlikely due to the genetic polymorphisms of the two genes since their respective sequences were identical in the clinical P. vivax isolates. Conclusion: PvSOP26 could be a promising TBV candidate for P. vivax, which warrants further evaluation. Graphical Abstract: [Figure not available: see fulltext.] |
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Faculty of Tropical Medicine, Mahidol University |
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Faculty of Tropical Medicine, Mahidol University Yongzhe Zhang Fei Liu Yan Zhao Fan Yang Jie Bai Xitong Jia Wanlapa Roobsoong Jetsumon Sattabongkot Liwang Cui Yaming Cao Enjie Luo Meilian Wang |
format |
Article |
author |
Yongzhe Zhang Fei Liu Yan Zhao Fan Yang Jie Bai Xitong Jia Wanlapa Roobsoong Jetsumon Sattabongkot Liwang Cui Yaming Cao Enjie Luo Meilian Wang |
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Yongzhe Zhang |
title |
Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates |
title_short |
Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates |
title_full |
Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates |
title_fullStr |
Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates |
title_full_unstemmed |
Evaluation of two Plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates |
title_sort |
evaluation of two plasmodium vivax sexual stage antigens as transmission-blocking vaccine candidates |
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2022 |
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https://repository.li.mahidol.ac.th/handle/123456789/77155 |
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1763494202354696192 |