The MTT assay application to measure the viability of spermatozoa: A variety of the assay protocols

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay is one of the methods used to evaluate the viability of sperm. In the assay, a tetrazolium component (MTT) is converted into MTT formazan by some specific enzymes in the viable cells. The amount of formazan produc...

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Bibliographic Details
Main Author: Kakanang Buranaamnuay
Other Authors: Mahidol University
Format: Review
Published: 2022
Subjects:
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/79161
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Institution: Mahidol University