Dengue virus in humans and mosquitoes and their molecular characteristics in northeastern Thailand 2016-2018

Dengue is hyperendemic in most Southeast Asian countries including Thailand, where all four dengue virus serotypes (DENV-1 to -4) have circulated over different periods and regions. Despite dengue cases being annually reported in all regions of Thailand, there is limited data on the relationship of...

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Main Authors: Patcharaporn Nonyong, Tipaya Ekalaksananan, Supranee Phanthanawiboon, Sirinart Aromseree, Juthamas Phadungsombat, Emi E. Nakayama, Tatsuo Shioda, Vorthon Sawaswong, Sunchai Payungporn, Kesorn Thaewnongiew, Hans J. Overgaard, Michael J. Bangs, Neal Alexander, Chamsai Pientong
Other Authors: Faculty of Tropical Medicine, Mahidol University
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Published: 2022
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/79312
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spelling th-mahidol.793122022-08-04T18:40:04Z Dengue virus in humans and mosquitoes and their molecular characteristics in northeastern Thailand 2016-2018 Patcharaporn Nonyong Tipaya Ekalaksananan Supranee Phanthanawiboon Sirinart Aromseree Juthamas Phadungsombat Emi E. Nakayama Tatsuo Shioda Vorthon Sawaswong Sunchai Payungporn Kesorn Thaewnongiew Hans J. Overgaard Michael J. Bangs Neal Alexander Chamsai Pientong Faculty of Tropical Medicine, Mahidol University PT Freeport Indonesia London School of Hygiene & Tropical Medicine Chulalongkorn University Faculty of Medicine, Khon Kaen University Research Institute for Microbial Diseases Kasetsart University Khon Kaen University Thailand Ministry of Public Health Norges Miljø- og Biovitenskapelige Universitet Multidisciplinary Dengue is hyperendemic in most Southeast Asian countries including Thailand, where all four dengue virus serotypes (DENV-1 to -4) have circulated over different periods and regions. Despite dengue cases being annually reported in all regions of Thailand, there is limited data on the relationship of epidemic DENV infection between humans and mosquitoes, and about the dynamics of DENV during outbreaks in the northeastern region. The present study was conducted in this region to investigate the molecular epidemiology of DENV and explore the relationships of DENV infection in humans and in mosquitoes during 2016–2018. A total of 292 dengue suspected patients from 11 hospitals and 902 individual mosquitoes (at patient’s houses and neighboring houses) were recruited and investigated for DENV serotypes infection using PCR. A total of 103 patients and 149 individual mosquitoes were DENV -positive. Among patients, the predominant DENV serotypes in 2016 and 2018 were DENV-4 (74%) and DENV-3 (53%) respectively, whereas in 2017, DENV-1, -3 and -4 had similar prevalence (38%). Additionally, only 19% of DENV infections in humans and mosquitoes at surrounding houses were serotypically matched, while 81% of infections were serotypically mismatched, suggesting that mosquitoes outside the residence may be an important factor of endemic dengue transmission. Phylogenetic analyses based on envelope gene sequences showed the genotype I of both DENV-1 and DENV-4, and co-circulation of the Cosmopolitan and Asian I genotypes of DENV-2. These strains were closely related to concurrent strains in other parts of Thailand and also similar to strains in previous epidemiological profiles in Thailand and elsewhere in Southeast Asia. These findings highlight genomic data of DENV in this region and suggest that people’s movement in urban environments may result in mosquitoes far away from the residential area being key determinants of DENV epidemic dynamics. 2022-08-04T11:40:04Z 2022-08-04T11:40:04Z 2021-09-01 Article PLoS ONE. Vol.16, No.9 September 2021 (2021) 10.1371/journal.pone.0257460 19326203 2-s2.0-85114881634 https://repository.li.mahidol.ac.th/handle/123456789/79312 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85114881634&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Multidisciplinary
spellingShingle Multidisciplinary
Patcharaporn Nonyong
Tipaya Ekalaksananan
Supranee Phanthanawiboon
Sirinart Aromseree
Juthamas Phadungsombat
Emi E. Nakayama
Tatsuo Shioda
Vorthon Sawaswong
Sunchai Payungporn
Kesorn Thaewnongiew
Hans J. Overgaard
Michael J. Bangs
Neal Alexander
Chamsai Pientong
Dengue virus in humans and mosquitoes and their molecular characteristics in northeastern Thailand 2016-2018
description Dengue is hyperendemic in most Southeast Asian countries including Thailand, where all four dengue virus serotypes (DENV-1 to -4) have circulated over different periods and regions. Despite dengue cases being annually reported in all regions of Thailand, there is limited data on the relationship of epidemic DENV infection between humans and mosquitoes, and about the dynamics of DENV during outbreaks in the northeastern region. The present study was conducted in this region to investigate the molecular epidemiology of DENV and explore the relationships of DENV infection in humans and in mosquitoes during 2016–2018. A total of 292 dengue suspected patients from 11 hospitals and 902 individual mosquitoes (at patient’s houses and neighboring houses) were recruited and investigated for DENV serotypes infection using PCR. A total of 103 patients and 149 individual mosquitoes were DENV -positive. Among patients, the predominant DENV serotypes in 2016 and 2018 were DENV-4 (74%) and DENV-3 (53%) respectively, whereas in 2017, DENV-1, -3 and -4 had similar prevalence (38%). Additionally, only 19% of DENV infections in humans and mosquitoes at surrounding houses were serotypically matched, while 81% of infections were serotypically mismatched, suggesting that mosquitoes outside the residence may be an important factor of endemic dengue transmission. Phylogenetic analyses based on envelope gene sequences showed the genotype I of both DENV-1 and DENV-4, and co-circulation of the Cosmopolitan and Asian I genotypes of DENV-2. These strains were closely related to concurrent strains in other parts of Thailand and also similar to strains in previous epidemiological profiles in Thailand and elsewhere in Southeast Asia. These findings highlight genomic data of DENV in this region and suggest that people’s movement in urban environments may result in mosquitoes far away from the residential area being key determinants of DENV epidemic dynamics.
author2 Faculty of Tropical Medicine, Mahidol University
author_facet Faculty of Tropical Medicine, Mahidol University
Patcharaporn Nonyong
Tipaya Ekalaksananan
Supranee Phanthanawiboon
Sirinart Aromseree
Juthamas Phadungsombat
Emi E. Nakayama
Tatsuo Shioda
Vorthon Sawaswong
Sunchai Payungporn
Kesorn Thaewnongiew
Hans J. Overgaard
Michael J. Bangs
Neal Alexander
Chamsai Pientong
format Article
author Patcharaporn Nonyong
Tipaya Ekalaksananan
Supranee Phanthanawiboon
Sirinart Aromseree
Juthamas Phadungsombat
Emi E. Nakayama
Tatsuo Shioda
Vorthon Sawaswong
Sunchai Payungporn
Kesorn Thaewnongiew
Hans J. Overgaard
Michael J. Bangs
Neal Alexander
Chamsai Pientong
author_sort Patcharaporn Nonyong
title Dengue virus in humans and mosquitoes and their molecular characteristics in northeastern Thailand 2016-2018
title_short Dengue virus in humans and mosquitoes and their molecular characteristics in northeastern Thailand 2016-2018
title_full Dengue virus in humans and mosquitoes and their molecular characteristics in northeastern Thailand 2016-2018
title_fullStr Dengue virus in humans and mosquitoes and their molecular characteristics in northeastern Thailand 2016-2018
title_full_unstemmed Dengue virus in humans and mosquitoes and their molecular characteristics in northeastern Thailand 2016-2018
title_sort dengue virus in humans and mosquitoes and their molecular characteristics in northeastern thailand 2016-2018
publishDate 2022
url https://repository.li.mahidol.ac.th/handle/123456789/79312
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