In vitro shoot organogenesis in Anthurium andraeanum 'HC 028' - a highly valued cut-flower and potted ornamental plant
A high-frequency clonal propagation protocol was developed for Anthurium andraeanum 'HC 028', a highly valued a cut-flower and potted ornamental plant. Shoot tip explants of Anthurium andraeanum 'HC 028' were cultured on MS medium fortified with different concentrations of BAP (1...
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| Format: | Conference or Workshop Item |
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2023
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| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/83274 |
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| Institution: | Mahidol University |
| Summary: | A high-frequency clonal propagation protocol was developed for Anthurium andraeanum 'HC 028', a highly valued a cut-flower and potted ornamental plant. Shoot tip explants of Anthurium andraeanum 'HC 028' were cultured on MS medium fortified with different concentrations of BAP (1.0-5.0 mg L-1) and kinetin (1.0-5.0 mg L-1) individually for shoot induction. The highest frequency (100%) of multiple shoot regeneration with maximum number of shoots (12.90 shoots explant-1) was noticed on MS medium supplemented with 4.0 mg L-1 BAP after being culture for 60 days. Elongated shoots dissected out from the in vitro proliferated shoot clumps were cultured on MS medium fortified with various concentrations (1.0-3.0 mg L-1) of auxins (NAA and IBA) for root induction. Highest frequency of rooting (100%) was noticed on MS medium augmented with 2.0 mg L-1 IBA. The plantlets were successfully acclimatized in the greenhouse with more than 95% survival rate. Regenerated plantlets were morphologically identical with mother plants. The present in vitro propagation protocol would facilitate an alternative method for rapid and large-scale production of Anthurium andraeanum 'HC 028' a cut-flower and potted ornamental plant. |
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