Molecular properties and ligand specificity of zebrafish intelectin-2
Intelectins are immune lectins expressed in chordates, including several fish species, in which intelectins are known to be upregulated upon infection. However, the basic biochemical properties and bacteria binding specificities of several fish intelectins are not well studied. We focus our investig...
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th-mahidol.832862023-06-18T23:37:26Z Molecular properties and ligand specificity of zebrafish intelectin-2 Singrang N. Mahidol University Agricultural and Biological Sciences Intelectins are immune lectins expressed in chordates, including several fish species, in which intelectins are known to be upregulated upon infection. However, the basic biochemical properties and bacteria binding specificities of several fish intelectins are not well studied. We focus our investigation on zebrafish intelectin-2 (DrIntL-2) that is predominantly expressed in the gastrointestinal tract. The disulfide-linked oligomeric state and the cysteine responsible for intermolecular disulfide bonds are identified. DrIntL-2 is a globular particle of around 30 nm. In addition to the typical exocyclic 1,2-diol ligands, DrIntL-2 binds β-1,3-glucan and recognizes Salmonella typhimurium and Pseudomonas aeruginosa. This investigation not only shed light on the fish innate immunity that will be essential for the aquaculture industry, but will also provide a foundation for further application of DrIntL-2 in bacteria detection and identification. 2023-06-18T16:37:26Z 2023-06-18T16:37:26Z 2022-04-01 Article Fish and Shellfish Immunology Vol.123 (2022) , 528-536 10.1016/j.fsi.2022.03.025 10959947 10504648 35337980 2-s2.0-85127317169 https://repository.li.mahidol.ac.th/handle/123456789/83286 SCOPUS |
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Agricultural and Biological Sciences Singrang N. Molecular properties and ligand specificity of zebrafish intelectin-2 |
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Intelectins are immune lectins expressed in chordates, including several fish species, in which intelectins are known to be upregulated upon infection. However, the basic biochemical properties and bacteria binding specificities of several fish intelectins are not well studied. We focus our investigation on zebrafish intelectin-2 (DrIntL-2) that is predominantly expressed in the gastrointestinal tract. The disulfide-linked oligomeric state and the cysteine responsible for intermolecular disulfide bonds are identified. DrIntL-2 is a globular particle of around 30 nm. In addition to the typical exocyclic 1,2-diol ligands, DrIntL-2 binds β-1,3-glucan and recognizes Salmonella typhimurium and Pseudomonas aeruginosa. This investigation not only shed light on the fish innate immunity that will be essential for the aquaculture industry, but will also provide a foundation for further application of DrIntL-2 in bacteria detection and identification. |
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Mahidol University |
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Mahidol University Singrang N. |
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Singrang N. |
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Singrang N. |
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Molecular properties and ligand specificity of zebrafish intelectin-2 |
title_short |
Molecular properties and ligand specificity of zebrafish intelectin-2 |
title_full |
Molecular properties and ligand specificity of zebrafish intelectin-2 |
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Molecular properties and ligand specificity of zebrafish intelectin-2 |
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Molecular properties and ligand specificity of zebrafish intelectin-2 |
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molecular properties and ligand specificity of zebrafish intelectin-2 |
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2023 |
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https://repository.li.mahidol.ac.th/handle/123456789/83286 |
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1781414570396483584 |