Phenotypic and functional changes of T cell subsets after CoronaVac vaccination
Background: The pandemic coronavirus disease 2019 (COVID-19) is a major global public health concern and several protective vaccines, or preventive/therapeutic approaches have been developed. Sinovac-CoronaVac, an inactivated whole virus vaccine, can protect against severe COVID-19 disease and hospi...
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th-mahidol.835562023-06-18T23:44:19Z Phenotypic and functional changes of T cell subsets after CoronaVac vaccination Phoksawat W. Mahidol University Biochemistry, Genetics and Molecular Biology Background: The pandemic coronavirus disease 2019 (COVID-19) is a major global public health concern and several protective vaccines, or preventive/therapeutic approaches have been developed. Sinovac-CoronaVac, an inactivated whole virus vaccine, can protect against severe COVID-19 disease and hospitalization, but less is known whether it elicits long-term T cell responses and provides prolonged protection. Methods: This is a longitudinal surveillance study of SARS-CoV-2 receptor binding domain (RBD)-specific IgG levels, neutralizing antibody levels (NAb), T cell subsets and activation, and memory B cells of 335 participants who received two doses of CoronaVac. SARS-CoV-2 RBD-specific IgG levels were measured by enzyme-linked immunosorbent assay (ELISA), while NAb were measured against two strains of SARS-CoV-2, the Wuhan and Delta variants. Activated T cells and subsets were identified by flow cytometry. Memory B and T cells were evaluated by enzyme-linked immune absorbent spot (ELISpot). Findings: Two doses of CoronaVac elicited serum anti-RBD antibody response, elevated B cells with NAb capacity and CD4+ T cell-, but not CD8+ T cell-responses. Among the CD4+ T cells, CoronaVac activated mainly Th2 (CD4+ T) cells. Serum antibody levels significantly declined three months after the second dose. Interpretation: CoronaVac mainly activated B cells but T cells, especially Th1 cells, were poorly activated. Activated T cells were mainly Th2 biased, demonstrating development of effector B cells but not long-lasting memory plasma cells. Taken together, these results suggest that protection with CoronaVac is short-lived and that a third booster dose of vaccine may improve protection. 2023-06-18T16:44:19Z 2023-06-18T16:44:19Z 2022-11-15 Article Vaccine Vol.40 No.48 (2022) , 6963-6970 10.1016/j.vaccine.2022.10.017 18732518 0264410X 36283898 2-s2.0-85140781068 https://repository.li.mahidol.ac.th/handle/123456789/83556 SCOPUS |
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Biochemistry, Genetics and Molecular Biology Phoksawat W. Phenotypic and functional changes of T cell subsets after CoronaVac vaccination |
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Background: The pandemic coronavirus disease 2019 (COVID-19) is a major global public health concern and several protective vaccines, or preventive/therapeutic approaches have been developed. Sinovac-CoronaVac, an inactivated whole virus vaccine, can protect against severe COVID-19 disease and hospitalization, but less is known whether it elicits long-term T cell responses and provides prolonged protection. Methods: This is a longitudinal surveillance study of SARS-CoV-2 receptor binding domain (RBD)-specific IgG levels, neutralizing antibody levels (NAb), T cell subsets and activation, and memory B cells of 335 participants who received two doses of CoronaVac. SARS-CoV-2 RBD-specific IgG levels were measured by enzyme-linked immunosorbent assay (ELISA), while NAb were measured against two strains of SARS-CoV-2, the Wuhan and Delta variants. Activated T cells and subsets were identified by flow cytometry. Memory B and T cells were evaluated by enzyme-linked immune absorbent spot (ELISpot). Findings: Two doses of CoronaVac elicited serum anti-RBD antibody response, elevated B cells with NAb capacity and CD4+ T cell-, but not CD8+ T cell-responses. Among the CD4+ T cells, CoronaVac activated mainly Th2 (CD4+ T) cells. Serum antibody levels significantly declined three months after the second dose. Interpretation: CoronaVac mainly activated B cells but T cells, especially Th1 cells, were poorly activated. Activated T cells were mainly Th2 biased, demonstrating development of effector B cells but not long-lasting memory plasma cells. Taken together, these results suggest that protection with CoronaVac is short-lived and that a third booster dose of vaccine may improve protection. |
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Mahidol University |
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Mahidol University Phoksawat W. |
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Phoksawat W. |
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Phoksawat W. |
title |
Phenotypic and functional changes of T cell subsets after CoronaVac vaccination |
title_short |
Phenotypic and functional changes of T cell subsets after CoronaVac vaccination |
title_full |
Phenotypic and functional changes of T cell subsets after CoronaVac vaccination |
title_fullStr |
Phenotypic and functional changes of T cell subsets after CoronaVac vaccination |
title_full_unstemmed |
Phenotypic and functional changes of T cell subsets after CoronaVac vaccination |
title_sort |
phenotypic and functional changes of t cell subsets after coronavac vaccination |
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2023 |
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https://repository.li.mahidol.ac.th/handle/123456789/83556 |
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