A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy

A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy

In the present study, a molecular beacon biosensor was developed to enable efficient detection of the viral RNAs using a previously described HyCaSD platform. The HyCaSD molecular beacon probes were labeled with the Cy5 and BHQ3 at each end of the hairpin probes. The fluorescent signal was detected...

Full description

Saved in:
Bibliographic Details
Main Author: Saisuk W.
Other Authors: Mahidol University
Format: Article
Published: 2023
Subjects:
Biochemistry, Genetics and Molecular Biology
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/83632
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Mahidol University
id th-mahidol.83632
record_format dspace
spelling th-mahidol.836322023-06-18T23:45:48Z A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy Saisuk W. Mahidol University Biochemistry, Genetics and Molecular Biology In the present study, a molecular beacon biosensor was developed to enable efficient detection of the viral RNAs using a previously described HyCaSD platform. The HyCaSD molecular beacon probes were labeled with the Cy5 and BHQ3 at each end of the hairpin probes. The fluorescent signal was detected immediately only when the molecular beacon probes specifically hybridized to the target sequence and unfolded their hairpin structures. This combination greatly improved the sensitivity with LOD of 100 copy equivalents per reaction (around 20-fold greater than the original HyCaSD). In addition, our MB-based HyCaSD demonstrated a single-step, single-tube and actual-time RNA detection procedure, thereby bringing it a major step closer to point-of-care diagnostic applications for viral infectious diseases. 2023-06-18T16:45:48Z 2023-06-18T16:45:48Z 2022-08-15 Article Analytica Chimica Acta Vol.1221 (2022) 10.1016/j.aca.2022.340134 18734324 00032670 35934369 2-s2.0-85133706132 https://repository.li.mahidol.ac.th/handle/123456789/83632 SCOPUS
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Saisuk W.
A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy
description In the present study, a molecular beacon biosensor was developed to enable efficient detection of the viral RNAs using a previously described HyCaSD platform. The HyCaSD molecular beacon probes were labeled with the Cy5 and BHQ3 at each end of the hairpin probes. The fluorescent signal was detected immediately only when the molecular beacon probes specifically hybridized to the target sequence and unfolded their hairpin structures. This combination greatly improved the sensitivity with LOD of 100 copy equivalents per reaction (around 20-fold greater than the original HyCaSD). In addition, our MB-based HyCaSD demonstrated a single-step, single-tube and actual-time RNA detection procedure, thereby bringing it a major step closer to point-of-care diagnostic applications for viral infectious diseases.
author2 Mahidol University
author_facet Mahidol University
Saisuk W.
format Article
author Saisuk W.
author_sort Saisuk W.
title A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy
title_short A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy
title_full A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy
title_fullStr A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy
title_full_unstemmed A molecular beacon biosensor for viral RNA detection based on HyCaSD strategy
title_sort molecular beacon biosensor for viral rna detection based on hycasd strategy
publishDate 2023
url https://repository.li.mahidol.ac.th/handle/123456789/83632
_version_ 1781414384123248640

Similar Items