Direct Sequencing of RNA and RNA Modification Identification Using Nanopore

Direct RNA sequencing (dRNA-seq) simultaneously enables the detection of RNA modifications and characterization of full-length transcripts. In principle, full-length native RNA molecule is translocated through the nanopore by a motor protein while a sensor measures ionic current shifts. Then, the cu...

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Bibliographic Details
Main Author:	Wongsurawat T.
Other Authors:	Mahidol University
Format:	Book Chapter
Published:	2023
Subjects:	Biochemistry, Genetics and Molecular Biology
Online Access:	https://repository.li.mahidol.ac.th/handle/123456789/83911
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Institution:	Mahidol University

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spelling	th-mahidol.839112023-06-18T23:50:29Z Direct Sequencing of RNA and RNA Modification Identification Using Nanopore Wongsurawat T. Mahidol University Biochemistry, Genetics and Molecular Biology Direct RNA sequencing (dRNA-seq) simultaneously enables the detection of RNA modifications and characterization of full-length transcripts. In principle, full-length native RNA molecule is translocated through the nanopore by a motor protein while a sensor measures ionic current shifts. Then, the current shifts are interpreted by an algorithm that turn out to RNA sequence. Currently, the standard protocol of dRNA-seq provided by Oxford Nanopore Technologies (ONT) allows to directly ligate and sequence only polyadenylated RNA (poly(A) RNA). Here, we describe a method of dRNA-seq that can be applied for both poly(A) RNA and non-poly(A) tailed-RNA. 2023-06-18T16:50:29Z 2023-06-18T16:50:29Z 2022-01-01 Book Chapter Methods in Molecular Biology Vol.2477 (2022) , 71-77 10.1007/978-1-0716-2257-5_5 19406029 10643745 35524112 2-s2.0-85129999197 https://repository.li.mahidol.ac.th/handle/123456789/83911 SCOPUS
institution	Mahidol University
building	Mahidol University Library
continent	Asia
country	Thailand Thailand
content_provider	Mahidol University Library
collection	Mahidol University Institutional Repository
topic	Biochemistry, Genetics and Molecular Biology
spellingShingle	Biochemistry, Genetics and Molecular Biology Wongsurawat T. Direct Sequencing of RNA and RNA Modification Identification Using Nanopore
description	Direct RNA sequencing (dRNA-seq) simultaneously enables the detection of RNA modifications and characterization of full-length transcripts. In principle, full-length native RNA molecule is translocated through the nanopore by a motor protein while a sensor measures ionic current shifts. Then, the current shifts are interpreted by an algorithm that turn out to RNA sequence. Currently, the standard protocol of dRNA-seq provided by Oxford Nanopore Technologies (ONT) allows to directly ligate and sequence only polyadenylated RNA (poly(A) RNA). Here, we describe a method of dRNA-seq that can be applied for both poly(A) RNA and non-poly(A) tailed-RNA.
author2	Mahidol University
author_facet	Mahidol University Wongsurawat T.
format	Book Chapter
author	Wongsurawat T.
author_sort	Wongsurawat T.
title	Direct Sequencing of RNA and RNA Modification Identification Using Nanopore
title_short	Direct Sequencing of RNA and RNA Modification Identification Using Nanopore
title_full	Direct Sequencing of RNA and RNA Modification Identification Using Nanopore
title_fullStr	Direct Sequencing of RNA and RNA Modification Identification Using Nanopore
title_full_unstemmed	Direct Sequencing of RNA and RNA Modification Identification Using Nanopore
title_sort	direct sequencing of rna and rna modification identification using nanopore
publishDate	2023
url	https://repository.li.mahidol.ac.th/handle/123456789/83911
_version_	1781415198725242880

Direct Sequencing of RNA and RNA Modification Identification Using Nanopore

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