Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2
Mouse antibodies specific to dengue NS1 have been widely investigated for their crossreactivity with several human biomolecules. This is the first study demonstrating the cross-reactivity of human monoclonal antibodies (HuMAbs) specific to dengue NS1 isolated from patients infected with dengue virus...
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th-mahidol.866832023-06-19T01:07:41Z Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2 Kowaboot S. Mahidol University Medicine Mouse antibodies specific to dengue NS1 have been widely investigated for their crossreactivity with several human biomolecules. This is the first study demonstrating the cross-reactivity of human monoclonal antibodies (HuMAbs) specific to dengue NS1 isolated from patients infected with dengue virus serotype-2 (DENV-2). Nine anti-NS1 HuMAbs, which were mainly derived from patients in convalescent-phase after secondary infection of DENV-2, were characterized. Their cross-reactivity with plasminogen, thrombin, and endothelial cells was investigated, following which plasmin-formation assays were performed. All anti-NS1 HuMAbs exhibited cross-reactivity with human plasminogen (Plg), but not with thrombin or endothelial cells. Moreover, all HuMAbs exhibiting cross-reactivity with Plg converted Plg to plasmin in the plasmin-formation assay. These results suggest the implications and drawbacks of using anti-NS1 antibodies in immunotherapy. 2023-06-18T18:07:41Z 2023-06-18T18:07:41Z 2022-01-01 Article Japanese Journal of Infectious Diseases Vol.75 No.1 (2022) , 24-30 10.7883/yoken.JJID.2020.1071 18842836 13446304 34053951 2-s2.0-85123813887 https://repository.li.mahidol.ac.th/handle/123456789/86683 SCOPUS |
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Medicine Kowaboot S. Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2 |
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Mouse antibodies specific to dengue NS1 have been widely investigated for their crossreactivity with several human biomolecules. This is the first study demonstrating the cross-reactivity of human monoclonal antibodies (HuMAbs) specific to dengue NS1 isolated from patients infected with dengue virus serotype-2 (DENV-2). Nine anti-NS1 HuMAbs, which were mainly derived from patients in convalescent-phase after secondary infection of DENV-2, were characterized. Their cross-reactivity with plasminogen, thrombin, and endothelial cells was investigated, following which plasmin-formation assays were performed. All anti-NS1 HuMAbs exhibited cross-reactivity with human plasminogen (Plg), but not with thrombin or endothelial cells. Moreover, all HuMAbs exhibiting cross-reactivity with Plg converted Plg to plasmin in the plasmin-formation assay. These results suggest the implications and drawbacks of using anti-NS1 antibodies in immunotherapy. |
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Mahidol University |
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Mahidol University Kowaboot S. |
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Kowaboot S. |
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Kowaboot S. |
title |
Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2 |
title_short |
Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2 |
title_full |
Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2 |
title_fullStr |
Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2 |
title_full_unstemmed |
Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2 |
title_sort |
characterization of human anti-dengue ns1 monoclonal antibodies derived from patients infected with denv-2 |
publishDate |
2023 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/86683 |
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1781415115002740736 |