Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2

A. marginale's major surface protein 2 (MSP2) is an immunodominant protein that is encoded by a multigene family. Phylogenetic analysis revealed that the msp2 sequence Thailand strain was clustered in third clade, with similarity values between 90.4 and 100%. The haplotype diversity showed 10 h...

Full description

Saved in:
Bibliographic Details
Main Author: Junsiri W.
Other Authors: Mahidol University
Format: Article
Published: 2023
Subjects:
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/87066
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Mahidol University
id th-mahidol.87066
record_format dspace
spelling th-mahidol.870662023-06-19T01:23:45Z Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2 Junsiri W. Mahidol University Veterinary A. marginale's major surface protein 2 (MSP2) is an immunodominant protein that is encoded by a multigene family. Phylogenetic analysis revealed that the msp2 sequence Thailand strain was clustered in third clade, with similarity values between 90.4 and 100%. The haplotype diversity showed 10 haplotypes of the msp2 genes. The entropy analysis of the nucleic and amino sequences revealed 289 and 117 high entropy peaks, respectively. Interestingly, one predicted allele belonging to MHC-II represented the hypervariable region (HVR) of MSP2. A. marginale's recombinant MSP2 (rAmMSP2), which has a molecular weight of 42 kDa, was examined in SDS-PAGE. Antigenicity of rAmMSP2 (42 kDa) and AmMSP2 (36 kDa) showed the conserved epitopes. The distribution of AmMSP2 on infected erythrocytes' membrane and outside was demonstrated by immunofluorescence detection. Therefore, the rMSP2 could be utilized in the establishment of immunodiagnostic tools and vaccine approaches for the monitoring of anaplasmosis. 2023-06-18T18:23:45Z 2023-06-18T18:23:45Z 2022-12-20 Article Research in Veterinary Science Vol.152 (2022) , 372-386 10.1016/j.rvsc.2022.08.019 15322661 00345288 36108550 2-s2.0-85137616172 https://repository.li.mahidol.ac.th/handle/123456789/87066 SCOPUS
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Veterinary
spellingShingle Veterinary
Junsiri W.
Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2
description A. marginale's major surface protein 2 (MSP2) is an immunodominant protein that is encoded by a multigene family. Phylogenetic analysis revealed that the msp2 sequence Thailand strain was clustered in third clade, with similarity values between 90.4 and 100%. The haplotype diversity showed 10 haplotypes of the msp2 genes. The entropy analysis of the nucleic and amino sequences revealed 289 and 117 high entropy peaks, respectively. Interestingly, one predicted allele belonging to MHC-II represented the hypervariable region (HVR) of MSP2. A. marginale's recombinant MSP2 (rAmMSP2), which has a molecular weight of 42 kDa, was examined in SDS-PAGE. Antigenicity of rAmMSP2 (42 kDa) and AmMSP2 (36 kDa) showed the conserved epitopes. The distribution of AmMSP2 on infected erythrocytes' membrane and outside was demonstrated by immunofluorescence detection. Therefore, the rMSP2 could be utilized in the establishment of immunodiagnostic tools and vaccine approaches for the monitoring of anaplasmosis.
author2 Mahidol University
author_facet Mahidol University
Junsiri W.
format Article
author Junsiri W.
author_sort Junsiri W.
title Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2
title_short Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2
title_full Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2
title_fullStr Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2
title_full_unstemmed Anaplasma marginale: Molecular discrimination, recombinant expression and characterization of major surface protein 2
title_sort anaplasma marginale: molecular discrimination, recombinant expression and characterization of major surface protein 2
publishDate 2023
url https://repository.li.mahidol.ac.th/handle/123456789/87066
_version_ 1781416415218106368