Targeted sequencing analysis pipeline for species identification of human pathogenic fungi using long-read nanopore sequencing

• Main Author: Langsiri N.
• Other Authors: Mahidol University
• Format: Article
• Published: 2023
• Subjects: Agricultural and Biological Sciences
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/90009

Among molecular-based techniques for fungal identification, Sanger sequencing of the primary universal fungal DNA barcode, the internal transcribed spacer (ITS) region (ITS1, 5.8S, ITS2), is commonly used in clinical routine laboratories due to its simplicity, universality, efficacy, and affordabili...