Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6)
Background and purpose: The use of fluoxetine raises the risk of pancreatic beta-cell dysfunction. However, the specific mechanism behind its mechanism of action in beta cells is unknown. This study investigated the cellular response of MIN6 cells to fluoxetine using untargeted cell-based metabolomi...
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th-mahidol.901992023-09-25T01:03:01Z Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6) Ngamratanapaiboon S. Mahidol University Pharmacology, Toxicology and Pharmaceutics Background and purpose: The use of fluoxetine raises the risk of pancreatic beta-cell dysfunction. However, the specific mechanism behind its mechanism of action in beta cells is unknown. This study investigated the cellular response of MIN6 cells to fluoxetine using untargeted cell-based metabolomics. Experimental approach: Metabolic profiling of MIN6 cells was performed using liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis on samples prepared under optimized conditions, followed by principal component analysis, partial least squares-discriminant analysis, and pair-wise orthogonal projections to latent structures discriminant analyses. Findings/Results: Sixty-six metabolites that had been differentially expressed between the control and fluoxetine-treated groups demonstrated that the citric acid cycle is mainly perturbed by fluoxetine treatment. Conclusion and implications: The current study provides insights into the molecular mechanisms of fluoxetine effects in MIN6 cells. 2023-09-24T18:03:01Z 2023-09-24T18:03:01Z 2023-09-01 Article Research in Pharmaceutical Sciences Vol.18 No.5 (2023) , 517-527 10.4103/1735-5362.383707 17359414 17355362 2-s2.0-85171366326 https://repository.li.mahidol.ac.th/handle/123456789/90199 SCOPUS |
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Pharmacology, Toxicology and Pharmaceutics Ngamratanapaiboon S. Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6) |
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Background and purpose: The use of fluoxetine raises the risk of pancreatic beta-cell dysfunction. However, the specific mechanism behind its mechanism of action in beta cells is unknown. This study investigated the cellular response of MIN6 cells to fluoxetine using untargeted cell-based metabolomics. Experimental approach: Metabolic profiling of MIN6 cells was performed using liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis on samples prepared under optimized conditions, followed by principal component analysis, partial least squares-discriminant analysis, and pair-wise orthogonal projections to latent structures discriminant analyses. Findings/Results: Sixty-six metabolites that had been differentially expressed between the control and fluoxetine-treated groups demonstrated that the citric acid cycle is mainly perturbed by fluoxetine treatment. Conclusion and implications: The current study provides insights into the molecular mechanisms of fluoxetine effects in MIN6 cells. |
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Mahidol University |
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Mahidol University Ngamratanapaiboon S. |
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Ngamratanapaiboon S. |
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Ngamratanapaiboon S. |
title |
Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6) |
title_short |
Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6) |
title_full |
Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6) |
title_fullStr |
Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6) |
title_full_unstemmed |
Metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (MIN6) |
title_sort |
metabolomic identification of biochemical changes induced by fluoxetine in an insulinoma cell line (min6) |
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2023 |
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https://repository.li.mahidol.ac.th/handle/123456789/90199 |
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1781414252730384384 |