Immunological detection of Salmonella paratyphi A in raw prawns

• Main Authors: S. Korbsrisate, S. Sarasombath, K. Janyapoon, P. Ekpo, S. Pongsunk
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Environmental Science; Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/9499

A slot blot enzyme-linked immunosorbent assay, using monoclonal antibodies specific only for Salmonella paratyphi A, to detect S. paratyphi A contamination in raw prawns has been established. When artificially contaminated prawn samples were tested, S. paratyphi A contamination could be identified correctly within 20 h. No false positives from samples artificially contaminated by other microorganisms were obtained. The sensitivity was such that as few as 1 S. paratyphi A organism per g of raw prawn could be detected. Therefore, the assay constituted a promising test for the rapid and specific detection of S. paratyphi A in prawns.