Immunological detection of Salmonella paratyphi A in raw prawns

A slot blot enzyme-linked immunosorbent assay, using monoclonal antibodies specific only for Salmonella paratyphi A, to detect S. paratyphi A contamination in raw prawns has been established. When artificially contaminated prawn samples were tested, S. paratyphi A contamination could be identified c...

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Main Authors: S. Korbsrisate, S. Sarasombath, K. Janyapoon, P. Ekpo, S. Pongsunk
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/9499
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spelling th-mahidol.94992018-02-27T11:26:48Z Immunological detection of Salmonella paratyphi A in raw prawns S. Korbsrisate S. Sarasombath K. Janyapoon P. Ekpo S. Pongsunk Mahidol University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Environmental Science Immunology and Microbiology A slot blot enzyme-linked immunosorbent assay, using monoclonal antibodies specific only for Salmonella paratyphi A, to detect S. paratyphi A contamination in raw prawns has been established. When artificially contaminated prawn samples were tested, S. paratyphi A contamination could be identified correctly within 20 h. No false positives from samples artificially contaminated by other microorganisms were obtained. The sensitivity was such that as few as 1 S. paratyphi A organism per g of raw prawn could be detected. Therefore, the assay constituted a promising test for the rapid and specific detection of S. paratyphi A in prawns. 2018-02-27T04:25:15Z 2018-02-27T04:25:15Z 1994-01-01 Article Applied and Environmental Microbiology. Vol.60, No.12 (1994), 4612-4613 00992240 2-s2.0-0028007540 https://repository.li.mahidol.ac.th/handle/123456789/9499 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0028007540&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Environmental Science
Immunology and Microbiology
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Environmental Science
Immunology and Microbiology
S. Korbsrisate
S. Sarasombath
K. Janyapoon
P. Ekpo
S. Pongsunk
Immunological detection of Salmonella paratyphi A in raw prawns
description A slot blot enzyme-linked immunosorbent assay, using monoclonal antibodies specific only for Salmonella paratyphi A, to detect S. paratyphi A contamination in raw prawns has been established. When artificially contaminated prawn samples were tested, S. paratyphi A contamination could be identified correctly within 20 h. No false positives from samples artificially contaminated by other microorganisms were obtained. The sensitivity was such that as few as 1 S. paratyphi A organism per g of raw prawn could be detected. Therefore, the assay constituted a promising test for the rapid and specific detection of S. paratyphi A in prawns.
author2 Mahidol University
author_facet Mahidol University
S. Korbsrisate
S. Sarasombath
K. Janyapoon
P. Ekpo
S. Pongsunk
format Article
author S. Korbsrisate
S. Sarasombath
K. Janyapoon
P. Ekpo
S. Pongsunk
author_sort S. Korbsrisate
title Immunological detection of Salmonella paratyphi A in raw prawns
title_short Immunological detection of Salmonella paratyphi A in raw prawns
title_full Immunological detection of Salmonella paratyphi A in raw prawns
title_fullStr Immunological detection of Salmonella paratyphi A in raw prawns
title_full_unstemmed Immunological detection of Salmonella paratyphi A in raw prawns
title_sort immunological detection of salmonella paratyphi a in raw prawns
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/9499
_version_ 1763493815162765312