Generalized and mobilizable positive-selection cloning vectors

• Main Authors: Skorn Mongkolsuk, Siritida Rabibhadana, Paiboon Vattanaviboon, Suvit Loprasert
• Other Authors: Chulabhorn Research Institute
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/9511
• Institution: Mahidol University

We have modified the positive-selection cloning vector pUN121 to expand the numbers of unique cloning sites by insertion of a multiple cloning site into the λ, ficI gene without disrupting its repressor function, resulting in plasmid pSKM10. Plasmid pSKM10 has seven restriction enzyme sites suitable...