Impaired Biliary Secretion of Immunoglobulin A in Vitamin A-Deficient Rats

• Main Authors: S. Puengtomwatanakul, S. Sirisinha
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/9670

The effect of vitamin A deficiency on biliary secretion of IgA was investigated. Rats used in this study were rendered vitamin A deficient following withdrawal of retinoic acid from the diet of retinoate-cycled animals. This procedure allows a precise control of both the onset of deficiency and dietary protein-energy input. Defective synthesis and transport of IgA antibodies into the bile was evident when vitamin A-deficient rats (A-) were immunized by injections of either Brucella abortus or sheep red blood cells directly into the Peyer's patches. Antibody titers in the bile of A- animals were significantly lower than those of A+ controls (P < 0.01). These A- rats also had significantly lower levels of total IgA in the bile compared with A+ controls (P < 0.05). Moreover, the transport of labeled rat IgA injected intravenously was adversely affected in these animals. These results, together with our previous report on the impaired intestinal antibody in A- rats, clearly indicate that vitamin A deficiency interferes with the transport of IgA antibodies into the bile of these animals. © 1986, SAGE Publications. All rights reserved.