Isolation of anoxygenic photosynthetic bacteria from Songkhla Lake for use in a two-staged biohydrogen production process from palm oil mill effluent
We are developing a process to produce biohydrogen from palm oil mill effluent. Part of this process will involve photohydrogen production from volatile fatty acids under low light conditions. We sought to isolate suitable bacteria for this purpose fromSongkhla Lake in Southern Thailand. Enrichme...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
ScienceDirect
2016
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| Subjects: | |
| Online Access: | http://kb.psu.ac.th/psukb/handle/2016/14979 |
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| Institution: | Prince of Songkhla University |
| Language: | English |
| Summary: | We are developing a process to produce biohydrogen from palm oil mill effluent. Part of
this process will involve photohydrogen production from volatile fatty acids under low
light conditions. We sought to isolate suitable bacteria for this purpose fromSongkhla Lake
in Southern Thailand. Enrichment for phototrophic bacteria from 34 samples was conducted
providing acetate as a major carbon source and applying culturing conditions of
anaerobic-low light (3000 lux) at 30 C. Among the independent isolates from these
enrichments 19 evolved hydrogen with productivities between 4 and 326 ml l 1 d 1. Isolate
TN1 was the most efficient producer at a rate of 1.85 mol H2 mol acetate 1 with a light
conversion efficiency of 1.07%. The maximum hydrogen production rate for TN1 was
determined to be 43 ml l 1 h 1. Environmentally desirable features of photohydrogen
production by TN1 included the absence of pH change in the cultures and no detectable
residual CO2 |
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