Electrochemical Biosensors Based on Molecularly Imprinted Polymer Biomimetic Receptors

Doctor of Philosophy (Chemistry), 2023

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Bibliographic Details
Main Author: Wardani, Nur Indah
Other Authors: Panote Thavarungkul
Format: Theses and Dissertations
Language:English
Published: Prince of Songkla University 2023
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Online Access:http://kb.psu.ac.th/psukb/handle/2016/19236
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Institution: Prince of Songkhla University
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spelling th-psu.2016-192362023-12-20T06:40:38Z Electrochemical Biosensors Based on Molecularly Imprinted Polymer Biomimetic Receptors Wardani, Nur Indah Panote Thavarungkul Faculty of Science (Chemistry) คณะวิทยาศาสตร์ ภาควิชาเคมี Electrochemical biosensor molecularly imprinted polymer Doctor of Philosophy (Chemistry), 2023 This thesis presents the utilization of molecularly imprinted polymer (MIP) biomimetic receptors as recognition elements in the development of two MIP electrochemical biosensors. One is a MIP cryogel for the direct detection of insulin, performed in a flow system. The other is an electrochemical biosensor with dual MIPs for the simultaneous determination of creatinine and albumin to provide the albumin to creatinine ratio (ACR) value. The insulin sensor was prepared using a gold electrode modified with carboxylated multiwalled carbon nanotubes (f-MWCNTs) to provide a large surface area platform for the high loading of the MIP cryogel and to increase the conductivity of the sensor. The MIP cryogel porous structure provided a large number of the imprinted recognition sites and improved the access of insulin to/from the MIP cavities. In addition, the flow system facilitated the mass transfer and limited the non-specific binding. This MIP cryogel provided a 0.050-1.40 pM linear range and a low limit of detection (LOD) of 33 fM with good stability at room temperature. For the dual MIP sensor, it was prepared on the dual screen-printed carbon electrodes (SPdCEs) modified with f-MWCNTs and redox probes, polymethylene blue (PMB) and ferrocene (Fc). The surface imprinting and electropolymerization were carried out to obtain more controlled imprinted binding sites of the two analytes on the respective electrode. This sensor was able to selectively recognize the two analytes with linear ranges of 5.0-100 ng mL-1 and 100-2500 ng mL-1 for creatinine and 5.0-100 ng mL-1 for albumin with an LOD of 1.5±0.2 ng mL-1 and 1.5±0.3 ng mL-1, respectively. The two MIP electrochemical biosensors exhibited good reusability and the real sample detection results showed comparable performances to the clinically employed standard methods (P  0.05). The good performances of these MIP electrochemical biosensors, i.e., high sensitivity and selectivity, low limit of detection, and high stability indicate their potential as alternative methods for analysis. 2023-12-20T06:40:22Z 2023-12-20T06:40:22Z 2023 Thesis http://kb.psu.ac.th/psukb/handle/2016/19236 en Attribution-NonCommercial-NoDerivs 3.0 Thailand http://creativecommons.org/licenses/by-nc-nd/3.0/th/ application/pdf Prince of Songkla University
institution Prince of Songkhla University
building Khunying Long Athakravi Sunthorn Learning Resources Center
continent Asia
country Thailand
Thailand
content_provider Khunying Long Athakravi Sunthorn Learning Resources Center
collection PSU Knowledge Bank
language English
topic Electrochemical biosensor
molecularly imprinted polymer
spellingShingle Electrochemical biosensor
molecularly imprinted polymer
Wardani, Nur Indah
Electrochemical Biosensors Based on Molecularly Imprinted Polymer Biomimetic Receptors
description Doctor of Philosophy (Chemistry), 2023
author2 Panote Thavarungkul
author_facet Panote Thavarungkul
Wardani, Nur Indah
format Theses and Dissertations
author Wardani, Nur Indah
author_sort Wardani, Nur Indah
title Electrochemical Biosensors Based on Molecularly Imprinted Polymer Biomimetic Receptors
title_short Electrochemical Biosensors Based on Molecularly Imprinted Polymer Biomimetic Receptors
title_full Electrochemical Biosensors Based on Molecularly Imprinted Polymer Biomimetic Receptors
title_fullStr Electrochemical Biosensors Based on Molecularly Imprinted Polymer Biomimetic Receptors
title_full_unstemmed Electrochemical Biosensors Based on Molecularly Imprinted Polymer Biomimetic Receptors
title_sort electrochemical biosensors based on molecularly imprinted polymer biomimetic receptors
publisher Prince of Songkla University
publishDate 2023
url http://kb.psu.ac.th/psukb/handle/2016/19236
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